Mfn2 sirna

Cells were seeded in 6-well clusters and then were incubated at 37 °C in 5% CO₂ until 80% confluent. Mst1 siRNA and Mfn2 siRNA were purchased from Santa Cruz, USA. siRNA transfection solution was prepared according to the directions provided by Santa Cruz to make a siRNA solution at a concentration of 400 nM. The cells were washed once with siRNA transfection medium (Santa Cruz, USA). Next, appropriate siRNA transfection medium and siRNA transfection solution were added to each well. The cells were then incubated for 6 h at 37 °C in 5% CO₂. The transfection mixture was then removed and replaced with normal growth medium, and then the cells were incubated for an additional 24 h. Western blotting was performed to observe the knockdown efficiency [32] (link).