Protocatechuate 3 4 dioxygenase pseudomonas pcd

Unmodified, dye-labeled, and biotinylated DNA oligonucleotides were purchased from MWG Eurofins. DNA scaffold strands were purchased from Tilibit (p7249, identical to M13mp18). Streptavidin (cat: S-888) and glass slides (cat: 10756991) were ordered from Thermo Fisher. Coverslips were purchased from Marienfeld (cat: 0107052). PEG-8000 was purchased from Merck (cat: 6510-1KG). Tris 1M pH 8.0 (cat: AM9856), EDTA 0.5M pH 8.0 (cat: AM9261), Magnesium 1M (cat: AM9530G) and Sodium Chloride 5M (cat: AM9759) were ordered from Ambion. Ultrapure water was obtained from a Milli-Q filter machine. Tween-20 (cat: P9416-50ML), Glycerol (cat: G5516-500ML), Methanol (cat: 32213-2.5L), BSA-Biotin (cat: A8549), Protocatechuate 3,4-Dioxygenase Pseudomonas (PCD) (cat: P8279), 3,4-Dihydroxybenzoic acid (PCA) (cat: 37580-25G-F) and (+-)-6-Hydroxy-2,5,7,8-tetra-methylchromane-2-carboxylic acid (Trolox) (cat: 238813-5G) were ordered from Sigma-Aldrich. Twinsil two-component glue was purchased from Picodent (cat: 13001000). Monoclonal antibodies against alpha-tubulin (cat: MA1-80017) was purchased from Thermo Scientific. The secondary antibodies Anti-Rat (cat: 712-005-150) were purchased from Jackson ImmunoResearch.

Unmodified DNA oligonucleotides, fluorescently modified DNA oligonucleotides, and biotinylated DNA oligonucleotides were purchased from MWG Eurofins. M13mp18 scaffold was obtained from Tilibit. BSA-Biotin was obtained from Sigma-Aldrich (cat: A8549). Streptavidin was ordered from Invitrogen (cat: S-888). Tris 1 M pH 8.0 (cat: AM9856), EDTA 0.5 M pH 8.0 (cat: AM9261), Magnesium 1 M (cat: AM9530G) and Sodium Chloride 5 M (cat: AM9759) were ordered from Ambion. Ultrapure water (cat: 10977-035) was purchased from Gibco. Polyethylene glycol (PEG)-8000 (catalog no. 6510-1KG) was purchased from Merck. Tween-20 (P9416-50ML), glycerol (65516-500 ml), methanol (32213-2.5 L), protocatechuate 3,4-dioxygenase pseudomonas (PCD; P8279), 3,4-dihydroxybenzoic acid (PCA; 37580-25G-F) and (±)-6-hydroxy- 2,5,7,8-tetra-methylchromane-2-carboxylic acid (trolox; 238813-5 G) were ordered from Sigma-Aldrich. Glass slides (cat: 48811-703) were obtained from VWR. Coverslips were purchased from Marienfeld (cat: 0107032). Silicon (cat.1300 1000) was ordered from picodent. Double-sided tape (cat: 665D) was ordered from Scotch.

Cy3B-modified and Atto643-modified DNA oligonucleotides were custom-ordered from Metabion. Sodium chloride 5 M (cat#: AM9759) was obtained from Ambion. Coverslips (cat#: 0107032) and glass slides (cat#: 10756991) were purchased from Marienfeld and Thermo Fisher. Double-sided tape (cat#: 665D) was ordered from Scotch. Two-component silica twinsil speed 22 (cat#: 1300 1002) was ordered from picodent. Glycerol (cat#: 65516-500ml), methanol (cat#: 32213-2.5L), protocatechuate 3,4-dioxygenase pseudomonas (PCD) (cat#: P8279), 3,4-dihydroxybenzoic acid (PCA) (cat#: 37580-25G-F) and (+−)–6-hydroxy-2,5,7,8- tetra-methylchromane-2-carboxylic acid (Trolox) (cat#: 238813-5G) were ordered from Sigma. Potassium chloride (cat#: 6781.1) was ordered from Carl Roth. Paraformaldehyde (cat#: 15710) was obtained from Electron Microscopy Sciences. 90 nm diameter Gold Nanoparticles (cat#: G-90-100) were ordered from Cytodiagnostics.