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Dcf da cellular reactive oxygen species detection assay kit

Manufactured by Beyotime
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The DCF-DA (2',7'-Dichlorofluorescin diacetate) Cellular Reactive Oxygen Species Detection Assay Kit is a fluorometric method for detecting and quantifying intracellular reactive oxygen species (ROS) in cell-based assays. The kit utilizes the cell-permeable dye DCF-DA, which becomes fluorescent upon oxidation by ROS.

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Eclipse ti, by Nikon (2 mentions)

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DCF-DA (43 citations) Reactive oxygen species detection kit (30 citations) Reactive oxygen detection kit (11 citations) Cellular Reactive Oxygen Species Detection Assay Kit (2 citations) DCF-DA kit (2 citations)

2 protocols using dcf da cellular reactive oxygen species detection assay kit

1

Intracellular ROS Detection Assay

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Intracellular ROS generation was detected by using a 2′,7′-dichlorofluorescin diacetate- (DCF-DA-) Cellular Reactive Oxygen Species Detection Assay Kit (Beyotime Institute of Biotechnology, Shanghai, China). Cells were incubated with 10 μM DCF-DA for 30 min in darkness at 37°C and washed three times with PBS. The fluorescence was observed under a fluorescent microscope (Eclipse Ti, Nikon, Melville, NY, USA) from six random fields and measured by Image J software (National Institutes of Health, Bethesda, MD, USA).
Zhang G.Y., Lu D., Duan S.F., Gao Y.R., Liu S.Y., Hong Y., Dong P.Z., Chen Y.G., Li T., Wang D.Y., Cheng X.S., He F., Wei J.S., Li G.Y., Zhang Q.Y., Wu D.D, & Ji X.Y. (2018). Hydrogen Sulfide Alleviates Lipopolysaccharide-Induced Diaphragm Dysfunction in Rats by Reducing Apoptosis and Inflammation through ROS/MAPK and TLR4/NF-κB Signaling Pathways. Oxidative Medicine and Cellular Longevity, 2018, 9647809.
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2

Intracellular ROS Measurement Using DCF-DA

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Intracellular ROS generation was measured by using a 2′,7′-dichlorofluorescin diacetate (DCF-DA)-Cellular Reactive Oxygen Species Detection Assay Kit (Beyotime Institute of Biotechnology, Shanghai, China). Cells were incubated with 10 μM DCF-DA for 30 min at 37 °C and washed three times with PBS. The fluorescence was observed by a fluorescent microscope (Eclipse Ti, Nikon, Melville, NY, USA) from five random fields and measured by ImageJ software (National Institutes of Health, Bethesda, MD, USA).
Wu D., Luo N., Wang L., Zhao Z., Bu H., Xu G., Yan Y., Che X., Jiao Z., Zhao T., Chen J., Ji A., Li Y, & Lee G.D. (2017). Hydrogen sulfide ameliorates chronic renal failure in rats by inhibiting apoptosis and inflammation through ROS/MAPK and NF-κB signaling pathways. Scientific Reports, 7, 455.
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