iPSCs were generated reprogramming fibroblasts from healthy controls and CMT2B patients carrying the RAB7V162M mutation by Sendai viral transduction of the transcription factors OCT4, SOX2, KLF4, and c-MYC (CytoTune2.0-iPS Sendai Reprogramming Kit, Thermo Fisher, Waltham, MS, USA). iPSCs were then tested for Sendai virus clearance (CytoTune2.0-iPS Sendai Reprogramming Kit, Thermo Fisher) and pluripotency (Trilineage differentiation Kit, Stem Cell Technologies, Vancouver, Canada). Cells were regularly screened and confirmed negative for mycoplasma during both maintenance and differentiation (MycoAlert kit, Lonza, Basel, Switzerland).
Cytotune2.0 ips sendai reprogramming kit
Manufactured by Thermo Fisher Scientific
Sourced in United States, Canada
The CytoTune2.0-iPS Sendai Reprogramming Kit is a laboratory equipment product designed for the generation of induced pluripotent stem (iPS) cells from various cell types. It utilizes Sendai virus vectors to deliver the Yamanaka transcription factors, enabling the reprogramming of somatic cells into a pluripotent state.
Automatically generated - may contain errors
Lab products found in correlation
1 protocol using cytotune2.0 ips sendai reprogramming kit
1
Generating patient-specific iPSCs
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!