Bilirubin total dpd gen 2 kit

Clinical chemistry routine parameters were available for each patient. Creatinine and bilirubin serum concentrations were determined by the application of enzymatic assay creatinine Plus Ver. 2 and Bilirubin Total DPD Gen.2 kit (both kits purchased from Roche, Mannheim, Germany), respectively according to manufacturer’s instructions. Both assays ran on the Cobas 6000 and 8000 analyzers (Roche, Mannheim, Germany). The RecombiPlasTin 2G kit (Instrumentation Laboratory, Lexington, USA) was used to determine the INR from citrated plasma using an ACL TOP 700 System (Instrumentation Laboratory, Lexington, USA).
MELD score was calculated according to the guidelines of the UNOS [12 ] using the following formula:
$MELD score=10 * \left(0.957 * ln\left(creatinine \left[mg/dl\right]\right)+0.378 * ln\left(bilirubin \left[mg/dl\right]\right)+1.12 * ln\left(INR\right) + 0.643\right)$
According to the guidelines, determined creatinine concentrations below 1.0 mg/dl or above 4.0 mg/dl were set on the defined minimum of 1.0 mg/dl or on the defined maximum of 4.0 mg/dl, respectively. The MELD score range is defined from 6 to 40.

NEFA, BHB, tBIL and AST activity were analyzed with an automatic analyzer for clinical chemistry (Cobas 6000/c501; Roche Diagnostics GmbH, Vienna, Austria) using standardized colorimetric enzymatic assays. NEFA was analyzed with the ASC-ACOD method (Wako Chemicals, Richmond, VA, USA, inter-day coefficient of variability (CV) < 0.75%, inter-day CV at 0.55 mmol/L = 0.75%, inter-day CV at 1.08 mmol/L = 4.91%). BHB was determined using the Ranbut method (Randox Laboratories Ltd., London, UK, inter-day CV = 0.57%, intra-day CV = 0.99%). tBIL was measured with the Bilirubin Total DPD Gen.2 Kit (Roche Diagnostics GmbH, Vienna, Austria, inter-day CV = 1.6%, intra-day CV = 2.6%). AST activity was analyzed by kinetic measurement of the enzyme activity with pyridoxal phosphate activation recommended by the International Federation of Clinical Chemistry (Roche Diagnostics GmbH, Vienna, Austria, inter-day CV = 0.6%, intra-day CV = 0.8%). All measurements were performed at the Clinical Pathology Platform of the Vetmeduni Vienna.