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Mouse anti cd63 mab

The Mouse anti-CD63 mAb is a monoclonal antibody that specifically binds to the CD63 antigen, which is a cell surface glycoprotein expressed on various cell types, including activated platelets, monocytes, and some cancer cells. This antibody can be used for the identification and characterization of cells expressing the CD63 antigen, but its specific applications and intended uses are not provided.

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2 protocols using mouse anti cd63 mab

1

Immunofluorescence and Western Blotting Protocols

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Mouse anti-Lamp1 mAb (H4A3; 1:500 for immunofluorescence or hereafter IF), mouse anti-CD63 mAb (H5C6; 1:100 for IF), and mouse anti-CD8a mAb (OKT8; 1:500 for IF) were from the Developmental Studies Hybridoma Bank. Mouse anti-GFP mAb (catalog no. sc-9996; 1:1000 for Western blotting) was purchased from Santa Cruz Biotechnology, Inc. Mouse anti-EEA1 mAb (catalog no. 610456; 1:500 for IF) and mouse anti-GM130 mAb (catalog no. 610823; 1:500 for IF) were from BD Biosciences. Horseradish peroxidase–conjugated goat anti-mouse (catalog no. 176516; 1:10,000 for Western blotting) was from Bio-Rad. Alexa Fluor–conjugated goat antibodies against mouse IgG (1:500 for IF) were from Thermo Fisher Scientific.
PNGase F was purchased from New England Biolabs. The following small molecules were commercially available: nocodazole (Merck; working concentration: 33 μm), brefeldin A (Epicenter Technologies; working concentration: 10 µg/ml), cycloheximide (Sigma–Aldrich; working concentration: 10 µg/ml), and GalNAc-O-Bn (Sigma–Aldrich; working concentration: 2 mm).
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2

Visualizing Lysosomal and Endosomal Markers

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Mouse anti-Lamp1 monoclonal antibody (mAb) (H4A3; 1:500 for immunofluorescence or hereafter IF), mouse anti-CD63 mAb (H5C6; 1:100 for IF), and mouse anti-CD8a mAb (OKT8; 1:500 for IF) were from Developmental Studies Hybridoma Bank. Mouse anti-GFP mAb (#sc-9996; 1:1000 for Western blot) was purchased from Santa Cruz. Mouse anti-EEA1 mAb (#610456; 1:500 for IF) and mouse anti-GM130 mAb (#610823; 1:500 for IF) were from BD Biosciences. Horseradish peroxidase (HRP)conjugated goat anti-mouse (#176516; 1:10,000 for Western blot) was from Bio-Rad. Alexa Fluorconjugated goat antibodies against mouse IgG (1:500 for IF) were from Thermo Fisher Scientific.
PNGase F was purchased from New England Biolab. The following small molecules were commercially available: nocodazole (Merck; working concentration: 33 µM), brefeldin A (Epicenter Technologies; working concentration: 10 µg/ml), cycloheximide (Sigma Aldrich; working concentration: 10 µg/ml), GalNAc-O-Bn (Sigma Aldrich; working concentration: 2 mM).
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