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1

Analytical Standards for Tea Metabolites

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Formic acid and methanol (LC-MS grade) were purchased from TIC (Tokyo, Japan) and Merck (Darmstadt, Germany), respectively. Deionized ultrapure water was obtained from Millipore (Bedford, MA, USA). Flavanols of epigallocatechin gallate (EGCG), epigallocatechin (EGC), epicatechin gallate (ECG), epicatechin (EC), gallocatechin gallate (GCG), gallocatechin (GC), catechin gallate (CG), and catechin (C), 1,3,7-trimethyluric acid, kaempferol-3-glucoside, quercetin-3-glucoside, quercetin-3-galactoside, myricetin 3-galactoside, naringenin, valine, tryptophan, arginine, proline, theogallin, chlorogenic acid and 5-methoxysalicylic acid were obtained from Sigma (St. Louis, MO, USA). Theacrine, theobromine and caffeine were purchased from Yuanye (Shanghai, China). Procyanidin B1, procyanidin B2 and procyanidin B3 were gained from ChemFaces (Wuhan, Hubei, China). Epiafzelechin and epiafzelechin 3-gallate were obtained from Mreda (Beijing, China) and Asiabio (Basel, Switzerland), respectively. epigallocatechin-3-O-(3″-O-methyl)-gallate (EGCG3″Me) and epicatechin-3-O-(3″-O-methyl)-gallate (ECG3″Me) were purchased from Nagara Science Co., Ltd. (Gifu, Japan). Phenylethyl primeveroside and benzyl primeveroside were synthesized by staff at the National Glycoengineering Research Center, Shandong University, Jinan, China (Li et al., 2019 (link)).
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2

Comprehensive Chemical Analysis of Teas

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Chromatographically pure acetonitrile and acetic acid was purchased from Thermo Fisher (Waltham, MA, USA). Catechins (C), epicatechin (EC), gallocatechin (GC), epigallocatechin (EGC), epigallocatechin gallate (ECG), gallocatechin gallate (GCG), epigallocatechin-3-O-gallate (EGCG), procyanidin B1, procyanidin C1, kaempferitrin, rutin, isoquercetin, myricetin-3-O-galactoside, myricetin-3-O-rutinoside, galloylglucose, gallic acid, cinnamic acid, chlorogenic acid, shikimic acid, p-coumaric acid, quinic acid, pyrogallic acid, theogallin, strictinin, theanine, theobromine, ethyl gallate, tannic acid, caffeine, L-sodium glutamate, and D-glucose were purchased from Yuanye (Shanghai, China). Quercetin, myricetin, kaempferol, and kaempferol-3-O-glucoside were purchased from Shanghai Zzstandard (Shanghai, China). Amino acid mixture standard reagent, borate buffer solution, o-phthalaldehyde (OPA), and 9-fluorene methyl chloroformate (FMOC) were purchased from Agilent Technology (Santa Clara, CA, USA). n-Hexane was purchased from Thermo Fisher (Waltham, MA, USA). 2-Methyl-3-heptanone and n-alkanes (C7–C30) were purchased from Sigma (St. Louis, MO, USA).
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