Zosuquidar hydrochloride

Ficoll Histopaque®-1077, newborn calf serum (NBCS), phytohaemagglutin (PHA), dimethylsulfoxide (DMSO), sodium fluoride (NaF), phenylmethylsulfonyl fluoride (PMSF), triton X-100, Fluorescein isothiocyanate-Dextran of 4-kDa (FD4), Rhodamine 123, Zosuquidar hydrochloride or LY-335979, protease and phosphatase inhibitor cocktails, and all reagent-grade chemicals for buffers were obtained from Sigma (Saint-Quentin Fallavier, France). The tissue digestion kit and myelin isolation beads were purchased from Miltenyi Biotech (Paris France). RPMI 1640 medium, DMEM, 5000 units of penicillin (base) and 5000 units of streptomycin (base)/mL mixture (PS), gentamycin, glutamine, trypsine-EDTA (0.05%), newborn calf serum, Quant-it protein assay from Gibco-Invitrogen (Fisher Scientific, Illkirch, France), EndoGRO-LS Complete Culture Media Kit, X-MAP® luminex Kit for cytokine/chemokine assay from Merck-Millipore (Saint Quentin Yvelines, France).

Sulforhodamine 101 (Sulfo101) was purchased from Chemodex (St. Gallen, Switzerland), 8-(2-[fluoresceinyl]aminoethylthio)adenosine-3′,5′-cyclic monophosphate (8-fluo-cAMP) was purchased from Biolog Life Science (Bremen, Germany), a fluorescent cyclosporine A derivative [N-ϵ(4-nitrobenzofurazan-7-yl)-D-Lys⁸]cyclosporine A (NBD-CsA) was synthesized as previously described (18 (link)), FA-polyethylene glycol PEG-2kDa-FITC was purchased from Biochempeg (Watertown, MA), and NHS-PEG₅-Mal and NHS-PEG₄₀-Mal were obtained from NOF (Tokyo, Japan). SAMSA fluorescein and NHS-TRITC were purchased from Thermo Fisher Scientific (Waltham, MA). Agarose, probenecid, erythromycin, verapamil hydrochloride, p-aminohippuric acid, MK-571 sodium salt hydrate, zosuquidar hydrochloride, saquinavir, indinavir sulfate salt hydrate, folic acid, fluorescein sodium salt (Fluo), FITC-dextran (40, 70, or 150 kDa), 1-phenyl-2-thiourea (PTU), and ethyl-3-aminobenzoate methanesulfonate (MS-222, tricaine) were purchased from Sigma-Aldrich (Buchs, Switzerland).