CD4+ T lymphocyte counts were determined from the patients’ whole blood by flow cytometry using BD FACSCalibur (BD, USA), according to the manufacturer’s instructions, and data were analyzed using Multiset™ (BD, USA). A quantitative analysis of HIV-1 RNA in plasma was performed according to the manufacturer’s instructions using Abbott M2000 RealTime HIV-1 assay (Abbott Molecular, Inc., Des Plaines, IL, USA).
Multiset
Manufactured by BD
Sourced in United States
Multiset is a piece of laboratory equipment designed for performing multiple simultaneous analyses. It facilitates the processing of multiple samples in parallel, allowing for increased efficiency and throughput in laboratory workflows.
Automatically generated - may contain errors
3 protocols using multiset
1
Quantitative Analysis of HIV-1
2
Flow Cytometry Immunophenotyping Protocol
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For the evaluation of lymphocyte subsets, peripheral blood was analyzed by Flow Cytometry in a 4-color BD FACS Calibur (BD, San Jose, Calif.), using the BD IMK kit with Trucount tubes (BD Biosciences) according to the manufacturer's instructions. An additional panel of monoclonal antibodies (including CD45RA, CD45RO, CD62L, and HLA-DR) was also performed for further characterization of T-cell differentiation, using a lyse-wash protocol. Finally, the IOTest Beta Mark kit (Immunotech SAS, a Beckman Coulter Company, Marseille, France) was also used for the characterization of the TCR Vβ repertoire.
Flow cytometry data analysis was performed using Multiset and CellQuestPro software (BD Biosciences).
Flow cytometry data analysis was performed using Multiset and CellQuestPro software (BD Biosciences).
3
Lymphocyte Subset Profiling by Flow Cytometry
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For the evaluation of lymphocyte subsets, peripheral blood was analyzed by flow cytometry in a 4-color BD FACS Calibur (BD, San Jose, CA), using the BD IMK kit with Trucount tubes (BD Biosciences) according to the manufacturer's instructions. An additional panel of monoclonal antibodies (including CD45RA, CD45RO, CD62L, and HLA-DR) was also performed for further characterization of T-cell differentiation, using a lyse-wash protocol. Finally, the IOTest Beta Mark kit (Immunotech SAS, a Beckman Coulter Company, Marseille, France) was also used for the characterization of the TCR Vβ repertoire.
For the liver biopsy, the sample was mechanically disaggregated in a Medicon chamber (BD Biosciences), recovered, and resuspended in phosphate buffered saline. The cell suspension was incubated with the BD IMK kit (BD Biosciences) in regular flow cytometry tubes, using a lyse-wash protocol.
Flow cytometry data analysis was performed using Multiset and CellQuest Pro software (BD Biosciences).
For the liver biopsy, the sample was mechanically disaggregated in a Medicon chamber (BD Biosciences), recovered, and resuspended in phosphate buffered saline. The cell suspension was incubated with the BD IMK kit (BD Biosciences) in regular flow cytometry tubes, using a lyse-wash protocol.
Flow cytometry data analysis was performed using Multiset and CellQuest Pro software (BD Biosciences).
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