Genomic DNA samples were extracted from 200 mg fecal samples using the Allprep PowerFecal DNA/RNA kit (Qiagen, Redwood City, California) according to the manufacturer's protocols. To reduce technical variability, the homogenization step was performed for all fecal samples in the same batch using a PowerLyzer24 instrument (Qiagen, Redwood City, California). DNA concentrations were measured by a Qubit 3.0 Fluorometer (Thermo Fisher Scientific, Waltham, Massachusetts), and the A260/A280 absorption ratios were assessed by a NanoDrop One C Microvolume Spectrophotometer (Thermo Scientific, Waltham, Massachusetts).
Powerlyzer24 instrument
Manufactured by Qiagen
The PowerLyzer24 instrument is a benchtop homogenizer designed for efficient disruption and lysis of a wide range of sample types, including tissue, plant, and microbial samples. The instrument utilizes bead-beating technology to effectively break down samples, preparing them for downstream processing and analysis.
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Lab products found in correlation
2 protocols using powerlyzer24 instrument
1
Fecal DNA Extraction for Microbiome Analysis
2
Fecal Sampling and Gut Microbiome Analysis
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Fecal samples were collected under sedation immediately after blood collection to prevent interference of epinephrine-mediated hyperglycemia (Table S1). Plastic fecal loops were coated with mineral oil and inserted into the rectum and descending colon of the cats until an adequate amount of feces was collected. The samples in this study reflect the fecal composition of the rectum and descending colon, which is representative of the lower gut. We referred to the microbiota characterized in the fecal samples in this research as cat gut microbiota.
Genomic DNA samples were extracted from 200 mg fecal samples using the Qiagen Allprep PowerFecal DNA/RNA kit (Qiagen, MD) following the manufacturer’s protocols. To achieve homogeneous results, the homogenization step was performed by the Qiagen PowerLyzer24 instrument (Qiagen, MD) in the same batch. DNA and total RNA concentrations were measured by a Qubit 3 Fluorometer (Invitrogen, CA), and the A260/A280 absorption ratios were assessed using a NanoDrop One C Microvolume Spectrophotometer (Thermo Fisher Scientific, MA).
Genomic DNA samples were extracted from 200 mg fecal samples using the Qiagen Allprep PowerFecal DNA/RNA kit (Qiagen, MD) following the manufacturer’s protocols. To achieve homogeneous results, the homogenization step was performed by the Qiagen PowerLyzer24 instrument (Qiagen, MD) in the same batch. DNA and total RNA concentrations were measured by a Qubit 3 Fluorometer (Invitrogen, CA), and the A260/A280 absorption ratios were assessed using a NanoDrop One C Microvolume Spectrophotometer (Thermo Fisher Scientific, MA).
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