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Celltrace am

Manufactured by Thermo Fisher Scientific
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CellTrace AM is a fluorescent dye used for cell labeling and tracking in cell-based assays. It covalently binds to cell surface proteins, allowing the monitoring of various cellular processes.

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Lab products found in correlation

Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Te300, by Nikon (2 mentions)

Spelling variants of this product

CellTrace calcein red-orange AM (26 citations) CellTrace™ calcein green AM (10 citations) CellTrace™ Calcein AM (3 citations) CellTrace calcein green AM dye (2 citations)

2 protocols using celltrace am

1

Astrocyte Oxidative Stress Assay

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Sensitivity of astrocytes to oxidative stress was assessed by exposing cultures to tBuOOH for 2 h or OGD for 1 h. Cells were stained with Hoechst 33342 (10 µg/ml, #H-3570; Life Technologies) to label all cell nuclei and/or calcein red-orange (CellTrace AM, 2 µM, #C34851; Life Technologies) to identify live cells that maintained their plasma membrane integrity. Cells with good morphology were counted as live and cells with beading were counted as dead cells. For pUltra virus-infected cells, cells with GFP expression represented viable cells. Images were acquired on an inverted microscope (TE300; Nikon). We calculated percent live cells as the number of viable cells divided by the total number of cells labeled with Hoechst 33342 (#H3570; Life technologies). We counted 5 random fields in every independent experiment, which was replicated a minimum of three times.
Chen Y., Holstein D.M., Aime S., Bollo M, & Lechleiter J.D. (2016). Calcineurin β protects brain after injury by activating the unfolded protein response. Neurobiology of disease, 94, 139-156.
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2

Astrocyte Oxidative Stress Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sensitivity of astrocytes to oxidative stress was assessed by exposing cultures to tBuOOH for 2 h or OGD for 1 h. Cells were stained with Hoechst 33342 (10 µg/ml, #H-3570; Life Technologies) to label all cell nuclei and/or calcein red-orange (CellTrace AM, 2 µM, #C34851; Life Technologies) to identify live cells that maintained their plasma membrane integrity. Cells with good morphology were counted as live and cells with beading were counted as dead cells. For pUltra virus-infected cells, cells with GFP expression represented viable cells. Images were acquired on an inverted microscope (TE300; Nikon). We calculated percent live cells as the number of viable cells divided by the total number of cells labeled with Hoechst 33342 (#H3570; Life technologies). We counted 5 random fields in every independent experiment, which was replicated a minimum of three times.
Chen Y., Holstein D.M., Aime S., Bollo M, & Lechleiter J.D. (2016). Calcineurin β protects brain after injury by activating the unfolded protein response. Neurobiology of disease, 94, 139-156.
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