First, cecal content was removed and homogenized. Then, pools of six animals from the same experimental group were prepared: 5 pools from day-old-chicks (30 samples), 10 pools from animals in CFC at mid-period (60 samples), 10 from animals in IFC at mid-period (60 samples), 10 pools from animals in CFC at the end of the growing period (60 samples) and 10 pools from animals in IFC at the end of the growing period (60 samples). The pools’ content was cultured directly onto a Coliform Chromogenic agar (Scharlab, S.L., Barcelona, Spain) in duplicate, and agar plates were incubated at 37 ± 1 °C for 24 h. After incubation, suspected colonies were streaked onto a nutrient medium (Scharlab, S.L., Barcelona, Spain) and incubated at 37 ± 1 °C for 24 h. Then, API-20E test (Biomerieux, S.L., Barcelona, Spain) was performed to confirm E. coli.
Coliform chromogenic agar
Manufactured by Scharlab
Sourced in Spain
Coliform Chromogenic agar is a selective and differential medium used for the detection and enumeration of coliform bacteria in water, food, and other samples. The agar contains chromogenic substrates that allow the differentiation of coliform bacteria based on their enzyme activity.
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Lab products found in correlation
2 protocols using coliform chromogenic agar
1
Escherichia coli Enumeration in Broiler
2
Isolation and Identification of E. coli
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Cecal content was removed and homogenized. Afterward, pools of 6 animals from each replica were prepared (5 pools/treatment), and the pools content was cultured directly onto a nonspecific medium: blood agar (Scharlab, S.L., Barcelona, Spain) in aerobic and anaerobic conditions, and 2 gram-negative specific media: MacConkey agar (Scharlab, S.L.) and Coliform chromogenic agar (Scharlab, S.L.). Agar plates were incubated at 37°C ± 1°C for 24 h. After incubation, suspected colonies were streaked into a nutrient medium (Scharlab, S.L.) and incubated at 37°C ± 1°C for 24 h. Then, API-20E test (Biomerieux, S.L.) was performed to confirm E. coli.
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