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Nupage gels

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The 4–15% NuPAGE gels are precast polyacrylamide gels designed for the separation and analysis of proteins. They have a gradient concentration of 4% to 15% acrylamide, which allows for the effective separation of a wide range of protein sizes.

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Lab products found in correlation

Polyvinylidene difluoride membrane, by Merck Group (2 mentions) Horseradish peroxidase conjugated igg, by Santa Cruz Biotechnology (2 mentions) Epiquik whole cell extraction kit, by Epigentek (2 mentions) Epiquik nuclear protein extraction kit, by Epigentek (2 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

NuPAGE 4–12% Bis-Tris gels (5 citations) NuPAGE Bis-Tris Gel (3 citations) NuPAGE 4–12% Bis-Tris Pre-cast gels (2 citations) NuPAGE precast gels (2 citations)

2 protocols using nupage gels

1

Western Blot Protein Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total protein or nuclear protein was extracted using the EpiQuik whole-cell extraction kit or EpiQuik nuclear protein extraction kit (Epigentek, USA). The protein concentration was measured following the manufacturer's instruction (Bio-Rad, USA). Protein was applied and separated on 4–15% NuPAGE gels (Bio-Rad) and transferred to polyvinylidene difluoride membranes (Millipore, USA). The membranes were blocked with 5% bovine serum albumin and incubated with specific antibodies (1:1,000 dilution) overnight. Horseradish peroxidase-conjugated IgG (1:10,000 dilution) from Santa Cruz Biotechnology (Santa Cruz, USA) was used to treat the membrane for 1 h, after which the membranes were enhanced with a SuperSignal West Pico Chemiluminescent Substrate (Thermo, USA). The relative amounts of the transferred proteins were quantified with scanning the autoradiographic films. Total protein or nuclear protein was normalized to the corresponding β-actin or PCNA level.
Zhang X., Li Y., Chen Y.E., Chen J, & Ma P.X. (2016). Cell-free 3D scaffold with two-stage delivery of miRNA-26a to regenerate critical-sized bone defects. Nature Communications, 7, 10376.
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2

Quantitative Western Blotting Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total protein or nuclear protein was extracted using the EpiQuik whole-cell extraction kit or EpiQuik nuclear protein extraction kit (Epigentek, USA). The protein concentration was measured following the manufacturer’s instruction (Bio-Rad, USA). Protein was applied and separated on 4–15% NuPAGE gels (Bio-Rad) and transferred to polyvinylidene difluoride membranes (Millipore, USA). The membranes were blocked with 5% bovine serum albumin and incubated with specific antibodies (1:1,000 dilution) overnight. Horseradish peroxidase-conjugated IgG (1:10,000 dilution) from Santa Cruz Biotechnology (Santa Cruz, USA) was used to treat the membrane for 1 h, after which the membranes were enhanced with a SuperSignal West Pico Chemiluminescent Substrate (Thermo, USA). The relative amounts of the transferred proteins were quantified with scanning the autoradiographic films. Total protein or nuclear protein was normalized to the corresponding β-actin or PCNA level.
Zhang X., Li Y., Chen Y.E., Chen J, & Ma P.X. (2016). Cell-free 3D scaffold with two-stage delivery of miRNA-26a to regenerate critical-sized bone defects. Nature Communications, 7, 10376.
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