Luciferase assay reagents were purchased from Promega (Madison, Wisconsin, USA). Filipin was purchased from APExBIO (Houston, USA). 25-HC, MTT, and Nile-red were acquired from Sigma-Aldrich (St. Louis, Missouri, USA). Lovastatin was purchased from Aladdin (Los Angeles, 19 CA, USA). KA, MG-132, and Cycloheximide were purchased from MedChem Express (Shanghai, China). FBS, F12K, and DMEM medium were purchased from GIBCO (New York, USA). Protein A/G beads were purchased from Santa Cruz (Dallas, Texas, USA).
Filipin
Manufactured by Apexbio
Sourced in United States, China
Filipin is a fluorescent dye that is commonly used in biological research. It selectively binds to cholesterol and is often used to visualize the distribution of cholesterol within cells and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Cycloheximide (chx), by MedChemExpress (1 mentions)
Nile red, by Merck Group (1 mentions)
Luciferase assay reagent, by Promega (1 mentions)
Protein a g bead, by Santa Cruz Biotechnology (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Mg132, by MedChemExpress (1 mentions)
Chlorpromazine, by Merck Group (1 mentions)
Galactose, by Merck Group (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Simvastatin, by Merck Group (1 mentions)
Tween 80, by Sinopharm (1 mentions)
Milli q water purifier, by Merck Group (1 mentions)
Galactosamine hydrochloride, by Merck Group (1 mentions)
Triethylamine, by Sinopharm (1 mentions)
2 protocols using filipin
1
Luciferase Assay and Lipid Metabolism
2
Lipid Nanoparticle Formulation for In Vitro Study
Check if the same lab product or an alternative is used in the 5 most similar protocols
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RES (transisomer) was purchased from Shaanxi Huike Botanical Development Co., Ltd. (Xi’an, People’s Republic of China). PLGA (DL-lactide:glycolide = 75:25, Mw = ~10,000) was obtained from RESENBio Technology Co., Ltd (Xi’an, People’s Republic of China). Galactosamine hydrochloride, galactose, LPS, chlorpromazine and simvastatin were purchased from Sigma-Aldrich (St Louis, MO, USA). Oleoyl chloride, triethylamine and Tween 80 were provided by Sinopharm Chemical Reagent (Shanghai Shi, People’s Republic of China). Filipin and 3-amino-6-chloro-N-(diaminomethylene)-5-(ethyl(isopropyl)amino)pyrazine-2-carboxamide (EIPA) were purchased from APExBIO (Shanghai, China). Purified water was produced by a Milli-Q water purifier (Merck Millipore, MA, USA). All other chemicals were of analytical grade and used as provided.
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