Soybeans (Glycine max) were obtained from Mexico City in a local market. Pepsin from porcine gastric mucosa (EC 3.4.23.1; ≥250 units/mg solid), pancreatin from porcine pancreas (EC 232-468-9; 8 × USP), the tripeptide IPI, called diprotin A, human recombinant DPP-IV enzyme, porcine pancreatic α-amylase (EC 3.2.1.1), and rat intestine α-glucosidase (EC 3.2.1.20) were purchased from Sigma-Aldrich (Madrid, Spain). The Quantitative Colorimetric Peptide Assay kit was from Pierce™ (Rockford, IL, USA), and the chromogenic substrate (H-Gly-Pro-p-nitroaniline) was purchased from Enzo Life Sciences Inc. (Farmingdale, NY, USA).
Rat intestine α glucosidase
Manufactured by Merck Group
Sourced in Spain
Rat intestine α-glucosidase is a laboratory product that functions as an enzyme involved in the hydrolysis of α-1,4-glucosidic linkages in carbohydrates. It is derived from the small intestine of rats.
Automatically generated - may contain errors
Lab products found in correlation
Pancreatin from porcine pancreas, by Merck Group (2 mentions)
Pepsin from porcine gastric mucosa, by Merck Group (2 mentions)
Human recombinant dpp 4 enzyme, by Merck Group (1 mentions)
H gly pro p nitroaniline, by Enzo Life Sciences (1 mentions)
Porcine pancreatic α amylase, by Merck Group (1 mentions)
Porcine pancreatic lipase type 2, by Merck Group (1 mentions)
2 protocols using rat intestine α glucosidase
1
In Vitro Digestibility of Soybean
2
Lentil Protein Enzymatic Modification
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Lentil seeds (Lens culinaris Medik. var. Castellana) were purchased from Semillas Iglesias S.A. (Salamanca, Spain), milled (Moulinex, Allençon, France), passed through a 60-mesh sieve with 0.5 mm pore size and stored at 4 °C until use. A commercial food grade protease Savinase® 16 L (16 KNPU/g) was provided by Novozyme (Bagsvaerd, Denmark). L. plantarum CECT 748 was purchased from the Spanish Type Culture Collection (CECT, Valencia, Spain). MidiTrap™ G10 gel filtration columns were from GE Healthcare (Barcelona, Spain). Enzymes used including ACE (EC 3.4.15.1), rat intestine α-glucosidase (EC 3.2.1.20), porcine pancreatic lipase type II (EC 3.1.1.3) , pepsin from porcine gastric mucosa (EC 3.4.23.1) , pancreatin from porcine pancreas as well as other chemicals were purchased from Sigma-Aldrich (Madrid, Spain) unless otherwise stated.
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