N cadherin 32 n cadherin

Rabbit polyclonal antibodies against Psen1‐NTF (B19), Aph1a (B80.3), Nct (9C3), Pen‐2 (B126), and App C‐terminus (B63.3) have been described (Annaert et al, 2001; Esselens et al, 2004). Aph1bc (L82) was generated in the laboratory by immunizing rabbits with a QDKNFLLYNQRSR peptide. Aplp1 (W1CT) and Aplpl2 (W2CT) were a gift from D. Walsh, and Syndecan3 (2E9) was a gift from G. David. Commercially available antibodies were as follows: anti‐Psen2‐CTF (D30G3) from Cell Signaling, anti‐beta‐actin (A5441) from Sigma, Lrp1 (EPR3724) from Epitomics, Dcc (A20) and Nrg1 (F‐20) from Santa Cruz, and N‐cadherin (32/N‐cadherin) from BD. For immunohistochemistry, the same App‐CTFs were used (1:7,500), and further, NeuN (1:1,000, Chemicon Millipore clone A60 #MAB377, mouse monoclonal), Iba1 (1:900, Wako #019‐19741, rabbit polyclonal), and Gfap (1:1,000, Dako #Z0334, rabbit polyclonal). ELISA‐capturing antibodies were as follows: JRF/cAb40/28 for Aβ₄₀ and JRF/cAb42/26 for Aβ₄₂ from Janssen Pharmaceutica (Beerse, Belgium). Detection antibody huAB25‐HRPO was obtained from Janssen Pharmaceutica.

The following primary antibodies were used for immunoblotting: 1:1000 RAB35 (rabbit; Cell Signaling, 9690 S), 1:10,000 Actin [C4] (mouse; Merck-Millipore, MAB1501), 1:10,000 GAPDH [6C5] (mouse; Merck-Millipore, MAB374), 1:1000 contactin-2/TAG-1 (goat; R&D systems, AF4439), 1:1000 CHL1 (goat; R&D systems, AF2147), and 1:1000 N-cadherin [32/N-cadherin] (rabbit; BD, 610920). The following primary antibodies were used for immunostaining: 1:100 NeuN [A60] (mouse; Merck-Millipore, MAB377), 1:200 GFAP (rabbit, Frontier Institute, GFAP-Rb-Af800), 1:200 β-galactosidase (mouse, Promega, Z3781), 1:100 Cux1 (rabbit; Santa Cruz, sc13024), 1:100 Ctip2 (rat; Abcam, ab18465), 1:300 phosphorylated neurofilament [SMI-31] (mouse; Covance, SMI-31R), 1:500 Tbr2 (rabbit; Abcam, ab23345), 1:300 Pax6 (rabbit; BioLegend, PRB-278P), 1:200 phospho-Histone H3 (Ser10) [RR002] (rabbit; Cell Signaling, 9701), and 1:100 Nestin [Rat-401] (mouse; Invitrogen, 14-5843-82). The following secondary antibodies were used for immunostaining: goat anti-rat Alexa Fluor-488, donkey anti-mouse Alexa Fluor-488, donkey anti-rabbit Alexa Fluor-488, donkey anti-mouse Alexa Fluor-594, and donkey anti-rabbit Alexa Fluor-594 (all antibodies are from Life Technologies).

Anti-Rac1 (23A8) and anti-β-actin (MAB 1501) monoclonal antibodies were purchased from [Upstate Biotechnology and Chemicon International, respectively (now Merck KGaA, and its affiliates), Darmstadt, Germany]. Both the anti-JNK and anti-phospho-JNK (Thr183/Tyr185) polyclonal antibodies were purchased from R&D Systems, Inc. (Minneapolis, MN, USA). E-cadherin (36/E-Cadherin) and N-cadherin (32/N-Cadherin) were obtained from BD Biosciences (Becton Dickinson, Franklin Lakes, New Jersey, USA). Cytokeratin (AE1/3) and vimentin (V9) were purchased from Dako Agilent Technologies, Inc. (Santa Clara, CA, USA). Secondary antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). NSC23766 and SP600125 were purchased from Tocris Bioscience (Bristol, UK). The complementary DNA (cDNA) of the dominant negative N17Rac1 (Rac-DN), which encoded for a mutated amino acid 17 from Thr to Asn was provided by Dr. A. Hall (University College London, Laboratory for Molecular Cell Biology, UK). The expression plasmid was constructed as previously described [22 (link)].