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Model 1525 pump

Manufactured by YMC
Sourced in United States

The Model 1525 pump is a laboratory equipment designed for fluid transfer. It features a reliable and efficient pumping mechanism to move liquids from one location to another. The core function of this pump is to provide a controlled and consistent flow of fluids for various applications in a laboratory setting.

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2 protocols using model 1525 pump

1

HPLC Analysis of Mutant Compound Differences

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A 10 µL extract was injected into the HPLC system (Waters Inc., Milford, MA, USA), which contained a model 1525 pump, an ODS column (Pack ODS-A, 250 × 4.6 mm, 5 µm, YMC Co., Ltd., Japan), and a model 2489 UV detector. The gradient increased from 20% to 100% MeOH over 30 min and then was retained at 100% MeOH for 10 min. The fold differences of these compounds between the mutant and WT were calculated by HPLC peak area according to the following formula: [Area (Sample)—Area (Control)]/[Area (WT)–Area (Control)].
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2

HPLC Analysis of Organic Compounds

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The extracts were analyzed in a HPLC system (Waters Inc., USA), which contained a model 1525 pump, a model 2489 UV detector, and a HPLC column (Pack ODS-A, 250 × 4.6 mm, 5 μm, YMC Co., Ltd., Japan). The gradient increased from 10 to 100% MeOH over 30 min and was retained at 100% for 10 min.
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