A scanning electron microscope (SEM, JEOL JSM-5600) was utilized to examine the surface morphology of the samples. Samples were mounted on adhesive carbon pads placed on aluminum stubs. The sputter coating of the samples with gold was carried out using a Hummer® 6.2 Sputter System (Anatech Ltd, Springfield, VA). The SEM was operated at an accelerating voltage of 10 kV.
Hummer 6.2 sputter system
Manufactured by Anatech
Sourced in United States
The Hummer 6.2 Sputter System is a laboratory equipment used for thin film deposition. It utilizes a sputtering process to deposit a thin, uniform coating of materials onto various substrates. The system is designed to provide a controlled and consistent deposition environment for research and development applications.
Automatically generated - may contain errors
Lab products found in correlation
Jsm 5600, by JEOL (2 mentions)
Paraformaldehyde p 0840 53, by Thermo Fisher Scientific (2 mentions)
Jsm 6500f, by JEOL (2 mentions)
Inspect s scanning electron microscope, by Thermo Fisher Scientific (1 mentions)
Zetasizer zs90, by Malvern Panalytical (1 mentions)
Quanta 200 sem, by Thermo Fisher Scientific (1 mentions)
Su3500, by Hitachi (1 mentions)
Su3500 sem, by Hitachi (1 mentions)
Jsm 5900lv, by JEOL (1 mentions)
10 protocols using hummer 6.2 sputter system
1
Surface Morphology Analysis via SEM
2
Scanning Electron Microscopy of Enteric Tablets
3
Ultrastructural Analysis of Mosquito Ovarian Follicles
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The ovaries were dissected from mosquitoes injected with Fluc control dsRNA and EOF1 dsRNA at 96 h PBM. Each follicle was carefully separated from the ovaries in 1× PBS under a dissecting microscope. The mature follicles were fixed in 2.5% glutaraldehyde in 0.1 M PIPES for 1 h at room temperature and washed twice in PIPES. The follicles were then postfixed in 1% osmium tetroxide in PIPES for 1 h and washed twice in deionized water for 10 min each. The follicles were dehydrated with ETOH in water through a graded series for 10 min each in 10%, 30%, 50%, 70%, and 90% ETOH and 3 times 30 min each in 100% ETOH at room temperature. The samples were dried with hexamethyldisilazane (HMDS; Electron Microscopy Sciences, Hatfield, PA, USA) in ETOH through a graded series for 20 min each in 25%, 50%, 75%, and 100% HMDS at room temperature. The follicle samples were air dried under a fume hood overnight at room temperature for SEM analysis. The dried samples were metallized with gold using Hummer 6.2 Sputter System (Anatech USA, Union City, CA, USA). Inspect-S scanning electron microscope (FEI, Hillsboro, OR, USA) was used to compare the ultrastructural characteristics of the ovarian follicles of females injected with Fluc and EOF1 dsRNA.
4
Scanning Electron Microscopy of Textured Materials
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Surface morphology of textured materials was visualized by the means of a JEOL 5600LV (Jeol Inc.) scanning electron microscope (SEM). For visualization under SEM, samples were fixed to aluminum stubs with double-sided adhesive carbon conductive tape and subsequently sputter-coated with carbon using a Hummer 6.2 Sputter System (Anatech USA).
5
Particle and Nanofiber Characterization
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Particle size distributions were determined using a Zetasizer ZS90 (Malvern Instruments Inc., Westborough, MA) and confirmed with scanning electron microscope (SEM), as described below. Samples were sputter-coated with 10 nm of platinum (Hummer 6.2 Sputter System, Anatech USA, Union City, CA). Particle and nanofiber morphology were evaluated using a Quanta 200 SEM (FEI, Hillsboro, OR) with an acceleration voltage of 10 kV and sized using ImageJ software (National Institutes of Health, Bethesda, MD).
6
Morphological Characterization of Bevacizumab Powder
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To assess morphology, SEM images of bevacizumab spray-dried powders were obtained using a Hitachi SU3500 (Hitachi High Technologies America Inc., Schaumburg, IL, USA). A trace amount of sample was applied to double-sided carbon tape mounted on an aluminum stub. The sample was then sputter-coated with gold/palladium for 10 min at 15 to 20 mV using a Hummer® 6.2 Sputter System (Anatech Ltd., Battle Creek, MI, USA).
7
Visualizing Spray-Dried Formulations by SEM
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SEM images of spray-dried formulations were obtained using a Hitachi SU3500 SEM (Hitachi High Technologies America Inc., Schaumburg, IL, USA). Trace quantities of powder were applied to a double-sided carbon tape mounted on an aluminum stud. Samples were sputter coated with gold/palladium for approximately 6 min at 15–20 mA plasma current using a Hummer 6.2 Sputter System (Anatech+ Ltd., Battle Creek, MI, USA).
8
Structural Analysis of Cartilage and Chitosan Scaffolds
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The structure of scaffolds and human AC was investigated by scanning electron microscopy (SEM, JSM-6500F; JEOL, Tokyo, Japan) after enzymatic depletion of the proteoglycan moiety and chondrocytes in phosphate-buffered solutions (PBS, H15–002; PAA Laboratories, Pasching, Austria) containing 1 mg/ml bovine hyaluronidase (type I, H3506; Sigma-Aldrich) and 1 mg/ml trypsin (Trypsin-EDTA, T4174; Sigma-Aldrich) at 37 °C for three days. Specimens were then fixed with 4% paraformaldehyde (P/0840/53; Fisher Scientific, Loughborough, UK) in PBS for 4 hours at room temperature, rinsed with water and dehydrated in graded ethanol series.
Prior to SEM imaging, all chitosan/PEO scaffolds and cartilage samples were coated with a thin conducting layer of gold (∼10 nm) with a Hummer 6.2 Sputter System (Anatech, Union City, CA). Images were obtained using an emission current of 10 µA and an accelerating voltage of 10 kV.
Prior to SEM imaging, all chitosan/PEO scaffolds and cartilage samples were coated with a thin conducting layer of gold (∼10 nm) with a Hummer 6.2 Sputter System (Anatech, Union City, CA). Images were obtained using an emission current of 10 µA and an accelerating voltage of 10 kV.
9
Visualizing Cartilage Ultrastructure by SEM
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Scanning electron microscopy (SEM) was employed following enzymatic digestion of proteoglycans and cells of AC samples in phosphate-buffered solutions (PBS, H15-002; PAA Laboratories, Pasching, Austria) containing 1 mg/ml bovine hyaluronidase (type I, H3506; Sigma-Aldrich) and 1 mg/ml trypsin (Trypsin-EDTA, T4174; Sigma-Aldrich) at 37 C for three days. 38 Specimens were then Exed with 4% paraformaldehyde (P/0840/53; Fisher Scientific, Loughborough, UK) in PBS for 4 h at room temperature, rinsed with water and dehydrated in graded ethanol series. Prior to SEM imaging, all samples were gold coated with a Hummer 6.2 Sputter System (Anatech, Union City, CA). SEM (JSM-6500 F; JEOL, Tokyo, Japan) imaging at an accelerating voltage of 10 kV revealed the meshwork structure and the fibril width to a depth of $0.25.
10
Zebrafish Larval Otolith Structural Analysis
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