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2 protocols using isogentisin

1

Phytochemical Analysis of Medicinal Plants

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Ultrapure water was prepared using a Milli-Q water purification system (Millipore, France). HPβCD (97%) was obtained from Acros Organics. Folin–Ciocalteu′s phenol reagent, sodium carbonate, orthophosphoric acid, and methanol were purchased from Sigma–Aldrich Chemie GmbH (Munich, Germany), while HPLC-grade acetonitrile was purchased from Merck (Darmstadt, Germany). Gallic acid (Extrasynthese, Genay, France), gentiopicroside (ChromaDex, Los Angeles, CA, USA), and isogentisin (Phytolab, Vestenbergsgreuth, Germany) were used as standards.
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2

Characterization of Medicinal Plant Compounds

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Maltodextrin (MD) ) was provided from Davisco Foods International (Le Sueur, MN, USA), whey protein (WP) was provided from Polmlek (Raciąż, Poland), pectin was provided from CPKelco (Großenbrode, Germany), and corn starch was supplied from Production sector of the Institute for Medicinal Plants Research Dr. Josif Pančić. Gelatin was produced by Aleva, Novi Kneževac. Folin-Ciocalteu reagent, gallic acid, orthophosphoric acid, and sodium carbonate were purchased from Sigma-Aldrich Chemie GmbH (Munich, Germany). Ultra-pure water was prepared using a Milli-Q purification system (Millipore, France), and HPLC-grade acetonitrile was obtained from Merck (Darmstadt, Germany). Standards swertiamarin, sweroside, and gentiopicroside were purchased from ChromaDex, USA, isoorientin and isovitexin were from Extrasynthese (Cedex, France), and isogentisin was purchased from Phytolab (Germany).
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