Rabbit polyclonal anti-pS316-TTP antibody was generated with the synthesized oligo-peptide PRRLPIFNRI(p)SVSE (Pocono Rabbit Farm & Laboratory, USA). The antiserum was purified by affinity chromatography by first collecting antibody binding to a PRRLPIFNRI(p)SVSE-peptide column, and, after elution, collecting the flow-through from a subsequent unphosphorylated PRRLPIFNRISVSE-peptide column. Immunoblots were probed with the following antibodies at the indicated concentrations: rabbit polyclonal anti-FLAG (Millipore Sigma, F7425; 1:1,000), mouse monoclonal anti-FLAG M2 (Millipore Sigma, F1804; 1:1,000), rabbit polyclonal anti-DDX6 (Bethyl, A300-461A; 1:1,000), rabbit polyclonal anti-GIGYF2 (Santa Cruz Biotechnology, sc-134708; 1:50), mouse monoclonal anti-14-3-3 (Santa Cruz Biotechnology, sc-1657; 1:1,000), rabbit polyclonal anti-TTP (Sigma-Aldrich, T5327; 1:500), rabbit polyclonal anti-CNOT1 (Proteintech, 14276-1-AP; 1:200), rabbit polyclonal anti-CNOT9 (Proteintech, 22503-1-AP, 1:500), mouse monoclonal anti-GAPDH (Cell Signaling Technology, 97166, 1:1,000), rabbit polyclonal anti-EDC4 ((42 (link)); 1:200) rabbit polyclonal anti-pSer178-TTP ((11) a generous gift from Dr. Georg Stoecklin, Heidelberg University; 1:500).
Anti gigyf2
Manufactured by Santa Cruz Biotechnology
Anti-GIGYF2 is an antibody product offered by Santa Cruz Biotechnology. It is designed to detect and bind to the GIGYF2 protein. GIGYF2 is a protein involved in the regulation of cell signaling pathways. The antibody can be used in various laboratory techniques to study the expression and function of GIGYF2 in biological samples.
Automatically generated - may contain errors
2 protocols using anti gigyf2
1
Antibody Generation and Characterization
2
Rabbit Polyclonal Anti-pS316-TTP Antibody Production
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Rabbit polyclonal anti-pS316-TTP antibody was generated with the synthesized oligo peptide PRRLPIFNRI(p)SVSE (Pocono Rabbit Farm & Laboratory, USA). The antiserum was purified by affinity chromatography by first collecting antibody binding to a PRRLPIFNRI(p)SVSE-peptide column, and, after elution, collecting the flow-through from a subsequent unphosphorylated PRRLPIFNRISVSE-peptide column. Western blots were probed with the following antibodies at the indicated concentrations: rabbit polyclonal anti-FLAG (Millipore Sigma, F7425; 1:1,000), mouse monoclonal anti-FLAG M2 (Millipore Sigma, F1804; 1:1000), rabbit polyclonal anti-DDX6 (Bethyl, A300-461A; 1:1,000), rabbit polyclonal anti-GIGYF2 (Santa Cruz Biotechnology, sc-134708; 1:50), mouse monoclonal anti-14-3-3 (Santa Cruz Biotechnology, sc-1657; 1:1,000), rabbit polyclonal anti-TTP (Sigma-Aldrich, T5327; 1:500), rabbit polyclonal anti-CNOT1 (Proteintech, 14276-1-AP; 1:200), rabbit polyclonal anti-CNOT9 (Proteintech, 22503-1-AP, 1:500), mouse monoclonal anti-GAPDH (Cell Signaling Technology, 97166, 1:1,000), rabbit polyclonal anti-EDC4 ((42); 1:200) rabbit polyclonal anti-pSer178-TTP ((11) a generous gift from Dr. Georg Stoecklin, Heidelberg University; 1:500).
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