Mda assay kit

The production of MDA, a marker of lipid peroxidation, was determined to assess oxidative injury using a commercial MDA assay kit (Nanjing, China). Brie y, H9C2 cells were seeded in 6-well plates for 24 h prior to various treatments. Following treatment, H9C2 cells were washed with ice-cold PBS and incubated with RIPA lysis buffer (Nanjing, China) for 10 min at 4°C. The lysates were centrifuged at 12,000 rpm for 10 min at 4°C, and the supernatant were collected for detection of MDA according to the manufacturer's instructions. The determination of SOD (Nanjing, China) is the same as above. All operations were carried out following the manufacture's instructions.

Blood samples collected from mice were used to measure creatinine, blood urea nitrogen (BUN) and MDA levels. Samples of blood were harvested from mice with or without intraperitoneal injection of 20 mg/kg cisplatin after 3 days. The levels of creatinine and BUN in blood samples were measured using the Creatinine and BUN Assay Kit (Nanjing, China) according to the manufacturer's instructions. The MDA levels were detected using the MDA Assay Kit (Nanjing, China).