The ESCC cell lines ECA109 and KYSE150 and the human normal esophageal epithelial cell line Het-1A were purchased from the Shanghai Institute of Cell Biology (Chinese Academy of Sciences, Shanghai, China). All cells were cultured in RPMI-1640 medium supplemented with 10% FBS at 37°C. Cells were tested for mycoplasma (MycoAlert Mycoplasma Detection Kit, Lonza) and certified mycoplasma free.
Het 1a
Manufactured by Shanghai Institute of Biochemistry and Cell Biology
Sourced in China
The Het-1A is a laboratory instrument designed for cell culture applications. It is used for the in vitro culture and maintenance of cells, including immortalized cell lines and primary cells. The Het-1A provides a controlled environment for cell growth and proliferation, ensuring optimal conditions for cell health and experimental reproducibility.
Automatically generated - may contain errors
Lab products found in correlation
Eca109, by Shanghai Institute of Biochemistry and Cell Biology (1 mentions)
Kyse150, by Shanghai Institute of Biochemistry and Cell Biology (1 mentions)
Mycoalert mycoplasma detection kit, by Lonza (1 mentions)
Fetal bovine serum (fbs), by GE Healthcare (1 mentions)
Het 1a, by GE Healthcare (1 mentions)
Rpmi 1640, by GE Healthcare (1 mentions)
2 protocols using het 1a
1
Culturing Esophageal Cell Lines
2
Esophageal Cancer Cell Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Two cell lines (TE-1 and TE-2) that were established from a well or poorly differentiated human ESCC [21 (link)] and the human esophageal epithelial cell line Het-1A [22 (link)] were obtained from Shanghai Institute of Cell Biology (Shanghai, China). TE-1 and TE-2 cell lines were cultured in RPMI 1640 (GE Healthcare Life Sciences, Logan, Utah, USA) and Het-1A cells were cultured in BEGM (GE Healthcare Life Sciences, Logan, Utah, USA) supplemented with 10% fetal bovine serum (GE Healthcare Life Sciences, Logan, Utah, USA) at 37°C with humidified 5% CO2.
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