Atcc 700392

Manufactured by American Type Culture Collection

Sourced in United States

ATCC 700392 is a laboratory equipment product offered by American Type Culture Collection (ATCC). It is a device designed for the cultivation and maintenance of cell cultures. The core function of this product is to provide a controlled and stable environment for the growth and propagation of cells in vitro.

Automatically generated - may contain errors

Lab products found in correlation

Atcc 43504, by American Type Culture Collection (4 mentions) Blood agar base no 2, by Thermo Fisher Scientific (2 mentions) Atcc 700684, by American Type Culture Collection (2 mentions) Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Tri gas incubator, by Esco Lifesciences (1 mentions) Ges 1 cells, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Brucella agar plates, by BD (1 mentions) Milli q grade, by Merck Group (1 mentions) Mouse elisa kit, by Neobioscience (1 mentions) Bca protein assay kit, by Beyotime (1 mentions) Cell counting kit 8 cck 8, by Apexbio (1 mentions) H pylori strain atcc 43504, by American Type Culture Collection (1 mentions)

6 protocols using atcc 700392

Bacterial Culture Protocol for H. pylori and E. coli

Check if the same lab product or an alternative is used in the 5 most similar protocols

H. pylori reference strains ATCC 700392, ATCC 43504 (metronidazole-resistant), and ATCC 700684 (clarithromycin resistant) were purchased from the American Type Culture Collection (Rockville, MD, USA). The strains were routinely grown in Blood Agar Base No. 2 (OXOID, Basingstoke, UK) supplemented with 8% defibrinated horse blood (OXOID) in a humidified microaerobic incubator (85% N₂, 10% CO₂, 5% O₂) at 37 °C for 48–72 h. For certain experiments, bacteria were grown for 48–72 h at 37 °C in brain heart infusion broth (OXOID) supplemented with 4% foetal bovine serum (Gibco, Carlsbad, CA, USA).
Escherichia coli strain ATCC 25922 and Staphylococcus epidermidis strain ATCC 12228, obtained from the local culture collection of the Department of Microbiology, Preventive Medicine and Public Health of the University of Zaragoza (Spain), were used in some susceptibility evaluations. For this purpose, the strains were grown in Mueller-Hinton agar/broth (PanReac AppliChem, Barcelona, Spain) overnight at 37 °C.

González A., Casado J., Chueca E., Salillas S., Velázquez-Campoy A., Sancho J, & Lanas Á. (2020). Small Molecule Inhibitors of the Response Regulator ArsR Exhibit Bactericidal Activity against Helicobacter pylori. Microorganisms, 8(4), 503.

+ Open protocol

+ Expand

Helicobacter pylori Co-Culture Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

The standard HP strains ATCC 43504 and ATCC 700392, as well as GES-1 cells, were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). CS01 was generously granted by Professor Jing Liu (University of Shanghai for Science and Technology, Shanghai, China), and QYZ-003 and QYZ-004 were obtained from the Qingyuan Hospital of Traditional Chinese Medicine (Qingyuan, China). All HP strains were authenticated, cultured, and stored at the School of Pharmaceutical Sciences (Shenzhen, China), Sun Yat-sen University. The strains were cultured on a Columbia agar base supplemented with 5% sterile defibrinated sheep blood or in BHI broth containing 10% FBS, shaken at 150 rpm, and subsequently incubated at 37 °C in a tri-gas incubator (ESCO, Singapore) with a gas composition of 10% CO₂, 5% O₂, and 85% N₂ for a period of two to three days, as previously described [29 (link)]. GES-1 cells were cultured with RPMI 1640 medium containing 10% FBS. For co-culture, following overnight incubation of the GES1 cells on 24-well plates, HP was added to the cells at an MOI ratio of 100:1, along with varying concentrations of drugs, for a co-incubation period of 6 h before being used for subsequent experiments.

Ou L., Zhu Z., Hao Y., Li Q., Liu H., Chen Q., Peng C., Zhang C., Zou Y., Jia J., Li H., Wang Y., Su B., Lai Y., Chen M., Chen H., Feng Z., Zhang G, & Yao M. (2024). 1,3,6-Trigalloylglucose: A Novel Potent Anti-Helicobacter pylori Adhesion Agent Derived from Aqueous Extracts of Terminalia chebula Retz. Molecules, 29(5), 1161.

+ Open protocol

+ Expand

In Vitro and In Vivo Helicobacter pylori Cultivation

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

H. pylori reference strains ATCC 700392, ATCC 43504 (metronidazole-resistant), and ATCC 700684 (clarithromycin resistant) were purchased from the American Type Culture Collection and used in the in vitro antibacterial assays. The strains were grown in Blood Agar Base No.2 (OXOID) supplemented with 8% defibrinated horse blood (OXOID) in a humidified microaerobic incubator (85% N₂, 10% CO₂, 5% O₂) at 37 °C for 48–72 h. For certain experiments, bacteria were grown for 48–72 h at 37 °C in brain heart infusion broth (OXOID) supplemented with 4% fetal bovine serum (Gibco). For in vivo experiments, the CagA⁺ mouse colonizing strain pre-mouse Sydney Strain 1 (PMSS1) [20 (link),21 (link)], obtained from the French National Reference Center for Campylobacters & Helicobacters (www.cnrch.fr), was used. For infection inocula, H. pylori PMSS1 was grown on in-house selective Wilkins Chalgren agar plates [22 (link)] under microaerobic conditions and transferred to Brucella broth medium as described below.

González A., Casado J., Chueca E., Salillas S., Velázquez-Campoy A., Espinosa Angarica V., Bénejat L., Guignard J., Giese A., Sancho J., Lehours P, & Lanas Á. (2019). Repurposing Dihydropyridines for Treatment of Helicobacter pylori Infection. Pharmaceutics, 11(12), 681.

+ Open protocol

+ Expand

Isolation and Culture of H. pylori Strains

Check if the same lab product or an alternative is used in the 5 most similar protocols

H. pylori reference strains of ATCC 49503, ATCC 43504, ATCC 51932, and ATCC 700392 were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA). H. pylori SS1 strain was obtained from the Korean Type Culture Collection at Gyeongsang National University (Jinju, Korea). H. pylori were incubated on Brucella agar plates (BD Biosciences, Braintree, MA, USA) supplemented with 10% bovine serum (BRL Life Technologies, Grand Island, NY, USA) at 37 °C for 72 h under a humidified condition with 5% CO₂. The number of bacterial particles in the H. pylori suspension was set to McFarland 0.33 and cultured in Mueller–Hinton broth supplemented with 10% bovine serum at 37 °C for 72 h under a humidified condition with 5% CO₂. Gastric biopsy specimens for the isolation of H. pylori were collected at Yong-In Severance Hospital, Korea. H. pylori clinical strains were isolated from 46 patients undergoing gastroscopic examinations to confirm the infection of H. pylori.

Kim H.W., Woo H.J., Yang J.Y., Kim J.B, & Kim S.H. (2021). Hesperetin Inhibits Expression of Virulence Factors and Growth of Helicobacter pylori. International Journal of Molecular Sciences, 22(18), 10035.

+ Open protocol

+ Expand

Evaluating Anti-Helicobacter pylori Activity

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The anti-H. pylori activity was assessed against a strain of Helicobacter pylori (American Type Culture Collection, H.b., ATCC 700392) using a micro-well dilution method as previously described [57 (link)]. Clarithromycin was used as a positive control and exhibited an MIC of 1.31 µM.

Alahdal A.M., Asfour H.Z., Ahmed S.A., Noor A.O., Al-Abd A.M., Elfaky M.A, & Elhady S.S. (2018). Anti-Helicobacter, Antitubercular and Cytotoxic Activities of Scalaranes from the Red Sea Sponge Hyrtios erectus. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(4), 978.

+ Open protocol

+ Expand

HMME-Based Antimicrobial Phototherapy

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hematoporphyrin monomethyl ether (HMME) was obtained from Shanghai Yuanye Bio‐Technology Co., Ltd (China). Iron chlorides anhydrous (FeCl₃), methanol, triethylamine (TEA), N,N‐dimethylformamide (DMF), dihydroartemisinin (DHA), tannic acid (TA), methylene blue (MB), TMB were purchased from Aladdin Bio‐Chem Technology Co., Ltd (Shanghai, China). Cell counting kit‐8 (CCK‐8) was bought from APExBIO (USA). Simulated gastric fluid (SGF, USP) was purchased from Leagene Biotechnology (China). Brain‐heart infusion (BHI) and Columbia blood plates were obtained from Huan kai Guangzhou Microbial (China). Singlet oxygen sensor green (SOSG) and live/dead BacLight bacterial viability kit (L‐7012) were bought from Invitrogen (USA). BCA protein assay kit was purchased from Beyotime (China). Mouse ELISA kits were purchased from NeoBioscience Technology Co, Ltd (China). H. pylori strain ATCC 43504 and ATCC 700392 were obtained from American Type Culture Collection (ATCC, USA). LQ2# and CS01 were clinical isolates gifted by Prof. Meicun Yao (Sun Yat‐sen University, Shenzhen, China.). BALB/c female mice (6–8 weeks old) were purchased from Gempharmatech Co., Ltd (China). High‐purify water (Millipore Milli‐Q grade) with a resistivity of 18.2 MΩ was used in all the experiments. All reagents used in the experiments were analytical grade without further purification.

Yu J., Guo Z., Yan J., Bu C., Peng C., Li C., Mao R., Zhang J., Wang Z., Chen S., Yao M., Xie Z., Yang C., Yang Y.Y., Yuan P, & Ding X. (2023). Gastric Acid‐Responsive ROS Nanogenerators for Effective Treatment of Helicobacter pylori Infection without Disrupting Homeostasis of Intestinal Flora. Advanced Science, 10(20), 2206957.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!