Phd ultra 4400 syringe pump

Immediately after craniotomy (see Methods: Electrophysiology), a microdialysis probe (CMA 12 Microdialysis probe, Harvard Apparatus) was implanted in the AC using the stereotaxic coordinates (Paxinos and Watson, 2007 ): bregma AP, –4.5 mm; ML, –7 mm; DV, 4.5 mm. The pump rate was set at 0.09 mL/h (PHD ultra 4400 Syringe pump, Harvard Apparatus). Samples were manually collected and frozen at –80°C until analysis with high performance liquid chromatography (Reinhoud et al., 2013 (link)).

Synthesized polymers were dissolved in CH₂Cl₂ (Avantor Performance Materials Poland S.A., Gliwice, Poland) and then mixed with docetaxel or cabazitaxel (LC Laboratories, Woburn, MA, USA). The concentration of each solution prepared for electrospinning was optimized in order to obtain a structure with well-developed fibres. Different nonwovens were obtained with the various amount of the drug: 3–5% (w/w) (Table 1) using a TL-Pro-BM electrospinning unit (Tong Li Tech, Shenzen, China). The device was equipped with two high voltage power supplies. One of them, that generates positive electrical potential, was applied to the spinneret, while the second one, that generates negative potential, was applied to the fibers collector, in form of a steel mandrel of 27 mm diameter (rotating collector). Polymer solutions were dosed to the spinning nozzle through a PTFE capillary, by using a PHD Ultra 4400 syringe pump (Harvard Apparatus, Holliston, MA, USA). Nonwoven mats were produced according to process parameters, presented in Table 1.