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Human factor 8

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Human factor VIII is a laboratory-produced blood-clotting protein. It is a critical component in the process of blood coagulation.

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Lab products found in correlation

Biophen fviii c kit, by Hyphen Biomed (4 mentions) 3 methyl 1 phenyl 2 pyrazolin 5 one pmp, by Merck Group (2 mentions) Rhamnose rha, by Merck Group (1 mentions) Deae sepharose ff, by GE Healthcare (1 mentions) Superdex peptide 10 300 gl column, by GE Healthcare (1 mentions) Sepharose cl 6b, by GE Healthcare (1 mentions) N acetyl d galactosamine, by Merck Group (1 mentions) L fucose, by Merck Group (1 mentions) Bsa standard, by Merck Group (1 mentions) Millipore milli q plus system, by Merck Group (1 mentions)

4 protocols using human factor 8

1

Extraction and Characterization of L. japonica Compounds

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L. japonica was purchased from Xiapu County, Fujian Province, China. Deuterium oxide (D2O, 99.9% atom D), 3-Methyl-1-phenyl-2-pyrazolin-5-one (PMP), glucose (Glc), GlcA, Gal, galacturonic acid (GalA), Xyl, and rhamnose (Rha) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Bovine serum albumin (BSA), trifluoroacetic acid (TFA), heparin, Fuc, ribose (Rib), arabinose (Ara), and Man were purchased from Aladdin Chemical Reagent Co., Ltd. (Shanghai, China). DEAE-Sepharose FF was from GE Healthcare (Uppsala, Sweden). CaCl2 solution (0.05 M), TT, PT, and APTT assay kits, and coagulation control plasma were obtained from TICO GmbH (Hamburg, Germany). Biophen FVIII: C kit was purchased from Hyphen Biomed (Neuvillesur-Oise, France). LMWH (0.4 mL × 4000 AXaIU) was from Sanofi-Aventis (Paris, France). Human factor VIII was obtained from Bayer Healthcare LLC (Leverkusen, Germany). All other chemicals, such as HCl, NaOH, and NaCl, were of analytical grade.
Li T., Ma H., Li H., Tang H., Huang J., Wei S., Yuan Q., Shi X., Gao C., Mi S., Zhao L., Zhong S, & Liu Y. (2022). Physicochemical Properties and Anticoagulant Activity of Purified Heteropolysaccharides from Laminaria japonica. Molecules, 27(9), 3027.
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2

Extraction and Purification of Polysaccharides from Sea Cucumber

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Dried sea cucumber A. japonicus was purchased from Dalian, Liaoning Province, China. Amberlite FPA98Cl was from Rohm and Haas Company (USA). Sepharose CL-6B and Superdex Peptide 10/300 GL columns (10 mm × 300 mm) were from GE Healthcare Life Sciences (Uppsala, Sweden). Bio-Gel P-2, P4, P-6, and P-10 columns were from Bio-Rad Laboratories (USA). Papain (800 U/mg) was from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Standard SEC-Pullulan was from Sepax Technologies Inc. (Delaware, America). l-Rhamnose (Rha), d-galactose (Gal), d-galacturonic acid (GalA), d-glucose (Glc), GlcA, 3-methyl-1-phenyl-2-pyrazolin-5-one (PMP) were from Sigma Chemical Co. (St. Louis, MO, USA). d-Mannose (Man), l-arabinose (Ara), d-ribose (Rib), d-xylose (Xyl), GalNAc, and l-Fuc were from Aladdin Chemical Reagent Co., Ltd. (Shanghai, China). LMWH (Enoxaparin, 0.4 mL × 4000 AXaIU) was from Sanofi-Aventis (France). OSCS was from Serva Electrophoresis GmbH (Germany). ADP, kallikrein chromogenic substrate CS-31 (02) and FIXa chromogenic substrate CS-51 (09) were from Hyphen Biomed (France). The APTT, CaCl2, and coagulation control plasma were from TICO GmbH (Germany). Human factor VIII was from Bayer Healthcare LLC (Germany) Biophen FVIII: C kit was from Hyphen Biomed (France). Human factor XII was from Assaypro (USA). All other chemicals used were of analytical grade.
Guan R., Peng Y., Zhou L., Zheng W., Liu X., Wang P., Yuan Q., Gao N., Zhao L, & Zhao J. (2019). Precise Structure and Anticoagulant Activity of Fucosylated Glycosaminoglycan from Apostichopus japonicus: Analysis of Its Depolymerized Fragments. Marine Drugs, 17(4), 195.
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3

Purification and Characterization of Enoxaparin from Sea Cucumber

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Dried sea cucumber P. mollis was purchased from Guangzhou, China. Amberlite FPA98 anion-exchange resin was obtained from the Rohm and Haas Company (St. Louis, MO, USA). Deuterium oxide (D2O, 99.9% Atom D) was obtained from Sigma-Aldrich (Shanghai, China). LMWH (Enoxaparin, 0.4 mL × 4000 AXaIU) was obtained from Sanofi-Aventis (Paris, France). The activated partial thromboplastin time (APTT), prothrombin time (PT) and thrombin time (TT) reagents, and standard human plasma were obtained from Teco Medical (Neufahrn N.B., Germany). Both Biophen FVIII: C kit and Biophen Heparin Anti-IIa/Anti-Xa kits were obtained from Hyphen Biomed (Paris, France). Human factor VIII was from Bayer HealthCare LLC (Berlin, Germany). All other chemicals were of reagent grade and are commercially available.
Zheng W., Zhou L., Lin L., Cai Y., Sun H., Zhao L., Gao N., Yin R, & Zhao J. (2019). Physicochemical Characteristics and Anticoagulant Activities of the Polysaccharides from Sea Cucumber Pattalus mollis. Marine Drugs, 17(4), 198.
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4

Characterization of Sea Cucumber Oligosaccharides

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Dried sea cucumbers H. fuscopunctata Jaeger were purchased from local markets in Guangdong Province and Hainan Province, China. Five oligosaccharide standards from LFG-Na (HS5, pentasaccharide, Mw = 1506 Da; HS8, octasaccharide, Mw = 2462 Da; HS11, hendecasaccharide, Mw = 3417 Da; HS14, tetradecasaccharide, Mw = 4373 Da; HS17, heptadecasaccharide, Mw = 5328 Da) were obtained from Kunming Institute of Botany, Chinese Academy of Sciences. BSA standard was purchased from Sigma (St. Louis, MO, USA). The monosaccharides including L-fucose and N-acetyl-D-galactosamine were purchased from Sigma-Aldrich, and D-glucuronic acid was from J&K Scientific Ltd. (Beijing, China). 1-Phenyl-3-methyl-5-pyrazolone (PMP) was purchased from Xiya Reagnent Co. (Linyi, China). Amberlite FPA98Cl ion-exchange resin was purchased from Rohm and Haas Company (Philadelphia, PA, USA). Deuterium oxide (D2O, 99.9% Atom D) was obtained from Sigma-Aldrich. The activated partial thromboplastin time (APTT) kits, CaCl2 and standard human plasma were purchased from MDC Hemostasis (Neufahrn, Germany), BIOPHEN FVIII: C kit was from Hyphen Biomed (Neuville sur Oise, France). Human factor VIII was from Bayer HealthCare LLC. Deionized water was prepared by the Millipore Milli Q-Plus system (Millipore, Billerica, MA, USA). All other reagents were of analytical grade and obtained commercially.
Zheng S., Wang Y., Wu J., Wang S., Wei H., Zhang Y., Zhou J, & Shi Y. (2022). Critical Quality Control Methods for a Novel Anticoagulant Candidate LFG-Na by HPSEC-MALLS-RID and Bioactivity Assays. Molecules, 27(14), 4522.
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