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Mouse anti wt1 antibody

Manufactured by Santa Cruz Biotechnology
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Mouse anti-WT1 antibodies are a type of immunoglobulin produced in mice that specifically recognize and bind to the Wilms' Tumor 1 (WT1) protein. WT1 is a transcription factor that plays a crucial role in the development and regulation of various tissues, including the kidneys, gonads, and certain types of cancers.

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Lab products found in correlation

Alexa fluor 488 anti rabbit igg, by Thermo Fisher Scientific (2 mentions) Alexa fluor 568 anti mouse igg, by Thermo Fisher Scientific (2 mentions) Rabbit anti nephrin, by Santa Cruz Biotechnology (2 mentions) Aqua poly mount, by Polysciences (2 mentions) Horseradish peroxidase conjugated secondary antibody, by Santa Cruz Biotechnology (2 mentions) Rabbit anti mouse collagen 4, by Bio-Rad (2 mentions) Rat anti f4 80, by Bio-Rad (2 mentions) Rabbit anti n tyr antibody, by Santa Cruz Biotechnology (2 mentions) Sigma fast dab chromogenic tablets, by Merck Group (2 mentions)

Close spelling variants of this product

Anti-WT1 (18 citations) Anti-WT1 antibody (6 citations) Mouse anti-WT1 (4 citations)

4 protocols using mouse anti wt1 antibody

1

Immunohistochemical Analysis of Kidney Markers

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Kidney sections from these mice were prepared in an identical fashion. Immunostaining was performed using rabbit anti-podocalyxin (R&D Systems), rabbit anti-nephrin (a gift from Dr. Larry Holzman), and mouse anti-WT1 antibodies (Santa Cruz Biotechnology). After washing, sections were incubated with a fluorophore-linked secondary antibody (Alexa Fluor 488 anti-rabbit IgG and Alexa Fluor 568 anti-mouse IgG from Invitrogen). Slides were subsequently mounted in Aqua Poly/Mount (Polysciences Inc.) and images were acquired using AxioVision IIe microscope with a digital camera.
Zhong F., Wang W., Lee K., He J.C, & Chen N. (2016). Role of C/EBP-α in Adriamycin-induced podocyte injury. Scientific Reports, 6, 33520.
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2

Immunostaining of Kidney Sections

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Kidney sections from these mice were prepared in an identical fashion. Immunostaining was performed using rabbit anti-synaptopodin (Fitzgerald), rabbit anti-nephrin (a gift from Dr. Larry Holzman), and mouse anti-WT1 antibodies (Santa Cruz Biotechnology). After washing, sections were incubated with a fluorophore-linked secondary antibody (Alexa Fluor 488 anti-rabbit IgG and Alexa Fluor 568 anti-mouse IgG from Invitrogen). After staining, slides were mounted in Aqua Poly/Mount (Polysciences Inc.) and photographed under an AxioVision IIe microscope with a digital camera.
Zhong F., Chen H., Wei C., Zhang W., Li Z., Jain M.K., Chuang P.Y., Chen H., Wang Y., Mallipattu S.K, & He J.C. (2014). Reduced Krüppel-like Factor 2 expression may aggravate the endothelial injury of diabetic nephropathy. Kidney international, 87(2), 382-395.
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3

Evaluating Kidney Injury Biomarkers

Cited 1 time
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Immunohistochemistry staining for the evaluation of collagen IV and Nitro-tyrosine (N-Tyr) levels, as well as macrophage and podocyte numbers in uninjured and injured kidneys was performed on paraffin kidney sections as reported.29 (link), 66 (link)-68 (link) Briefly, kidney sections were incubated with rabbit anti-mouse collagen IV (1:500, AbD Serotec), rabbit anti-N-Tyr antibody (1:400, Santa Cruz), mouse anti-WT1 antibody (1:100, Santa Cruz), and rat anti-F4/80 (AbD Serotec) overnight at 4°C followed by 1 hour incubation with the appropriate horseradish peroxidase-conjugated secondary antibody (1:100, Santa Cruz) at room temperature. Slides were developed using Sigma Fast DAB chromogenic tablets (Sigma) and then counterstained with toluidine blue in order to increase the contrast with DAB staining.
Wang H., Chen X., Su Y., Paueksakon P., Hu W., Zhang M.Z., Harris R.C., Blackwell T.S., Zent R, & Pozzi A. (2015). p47phox contributes to albuminuria and kidney fibrosis in mice. Kidney international, 87(5), 948-962.
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4

Evaluating Kidney Injury Biomarkers

Cited 1 time
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Immunohistochemistry staining for the evaluation of collagen IV and Nitro-tyrosine (N-Tyr) levels, as well as macrophage and podocyte numbers in uninjured and injured kidneys was performed on paraffin kidney sections as reported.29 (link), 66 (link)-68 (link) Briefly, kidney sections were incubated with rabbit anti-mouse collagen IV (1:500, AbD Serotec), rabbit anti-N-Tyr antibody (1:400, Santa Cruz), mouse anti-WT1 antibody (1:100, Santa Cruz), and rat anti-F4/80 (AbD Serotec) overnight at 4°C followed by 1 hour incubation with the appropriate horseradish peroxidase-conjugated secondary antibody (1:100, Santa Cruz) at room temperature. Slides were developed using Sigma Fast DAB chromogenic tablets (Sigma) and then counterstained with toluidine blue in order to increase the contrast with DAB staining.
Wang H., Chen X., Su Y., Paueksakon P., Hu W., Zhang M.Z., Harris R.C., Blackwell T.S., Zent R, & Pozzi A. (2015). p47phox contributes to albuminuria and kidney fibrosis in mice. Kidney international, 87(5), 948-962.
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