Chicken anti rabbit fluorescein isothiocyanate conjugated igg

Rabbit anti-S6, rabbit anti-phospho-S6 (ser235, 236), rabbit anti-4EBP-1, rabbit anti-phospho-4EBP-1 (Thr37, 46), rabbit anti-β-Catenin (species cross-reactivity: human, mouse, and rat) and mouse anti-proliferating cell nuclear antigen (PCNA) were from Cell Signaling Technology (Beverly, MA). Rabbit anti-α-Amylase was from Sigma Chemical Co. (St. Louis, MO). Mouse anti-CA19-9 was from Origene (Beijing, China). Goat anti-insulin A (C-12) (species cross-reactivity: human, mouse, and rat), Goat anti-glucagon (N-17) (species cross-reactivity: human, mouse, and rat), rabbit anti-somatostatin (FL-116) (species cross-reactivity: human, mouse, and rat), goat anti-rabbit fluoresceinisothiocyanate-conjugated IgG, donkey anti-goat fluoresceinisothiocyanate- conjugated IgG, donkey anti-goat Texas Red-conjugated IgG, goat anti-mouse Texas Red-conjugated IgG, and chicken anti-rabbit fluoresceinisothiocyanate-conjugated IgG and rapamycin were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). Dimethylsulfoxide was from Sigma Chemical Co. (St. Louis, MO). Aprotinin was purchased from Amersham Biosciences (Pittsburgh, PA). IRDye-conjugated affinity purified anti-rabbit, anti-mouse IgGs were purchased from Rockland (Gilbertsville, PA).

DAG was purchased from Phoenix Pharmaceuticals Inc. (Burlingame, CA, USA). Rabbit phospho-AKT (Ser473), phospho-S6, protein kinase B (AKT), S6, phosphatase and tensin homolog (PTEN), and PPARγ antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Rosiglitazone, rapamycin, leucine, rat lysosomal-associated membrane protein 2 (LAMP2, Mac3) antibody, and chicken anti-rabbit fluorescein isothiocyanate-conjugated IgG were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). DyLigh 594-goat anti-rat IgG was purchased from Abbkine Scientific Co (Wuhan, China).