Spirulina platensis powder was purchased from Qingdao Haixingyuan Biotechnology Co. Ltd. (Qingdao, China) The powder was washed thrice with distilled water to eliminate impurities and collected by centrifugation at 5000 rpm for 10 min. The washed powder was then dried overnight at 60 °C. The dried powder was pulverized and sieved using a standard sieve with a 200 mesh size. Sodium alginate (90%, SA) and calcium chloride (99%, CaCl2·2H2O) were obtained from Duksan Pure Chemicals (Ansan, Republic of Korea) and used to prepare alginate beads via Ca2+ crosslinking. Lead nitrate (99%, Pb(NO3)2), copper nitrate trihydrate (99%, Cu(NO3)2·3H2O), zinc nitrate hexahydrate (98%, Zn(NO3)2·6H2O), and cadmium nitrate tetrahydrate (98%, Cd(NO3)2·4H2O) were obtained from Daejung Chemical (Busan, Republic of Korea).
Calcium chloride
Manufactured by Duksan Pure Chemicals
Calcium chloride is an inorganic compound with the chemical formula CaCl2. It is a white, crystalline solid that is highly soluble in water. Calcium chloride is commonly used as a desiccant, a road de-icer, and in various industrial and laboratory applications.
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2 protocols using calcium chloride
1
Spirulina-based Alginate Beads for Heavy Metal Removal
2
Detailed Reagent Sourcing for Biomedical Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Sodium chloride was purchased from Tomita Pharmaceutical Co., Ltd. (Tokushima, Japan). Potassium chloride, calcium chloride, and magnesium chloride were obtained from Duksan Pure Chemicals Co. (Gyeonggi-do, Korea). Lactoperoxidase and glucose oxidase were provided from Biopole (Les Isnes, Belgium) and Amano enzyme Inc. (Nagoya, Japan), respectively. Lactoferrin and lysozyme were purchased from Tatua (Morrinsville, New Zealand) and Dalian Greensnow Egg Products Development Co., Ltd. (Dalian City, People’s Republic of China), respectively. Xylitol and potassium thiocyanate were supplied from Namyung Commercial Co., Ltd. (Seoul, Korea) and Friends Union Enterprises Ltd. (Tianjin, People’s Republic of China), respectively. Carbopol 934P and HEC hhx250 were provided from Lubrizol Corporation (Wickliffe, OH, USA) and Hercules Inc. (Wilmington, DE, USA), respectively. Streptozotocin was purchased from Sigma-Aldrich Chemical Co. (St Louis, MO, USA). All other reagents were of analytical grade and were used without further purification. Double-distilled water was used for all experiments.
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