Lipofectaine 2000 reagent

Manufactured by Thermo Fisher Scientific

Sourced in United States

Lipofectamine 2000 reagent is a cationic lipid-based transfection reagent used for the delivery of nucleic acids, including DNA and RNA, into a variety of cell types. It facilitates the uptake of genetic materials into the target cells by forming complexes with the nucleic acids. The reagent can be used for gene expression, gene silencing, and other applications that require the introduction of genetic material into cells.

Automatically generated - may contain errors

Lab products found in correlation

Control mimic, by Qiagen (2 mentions) Red siglo oligos, by Thermo Fisher Scientific (2 mentions)

2 protocols using lipofectaine 2000 reagent

Transient Transfection of Viral miRNA Mimics

Check if the same lab product or an alternative is used in the 5 most similar protocols

Transient transfections were performed using Lipofectaine 2000 reagent (Life Technologies, San Diego, CA, USA) according to manufacturer’s instructions. Red siGLO oligos (Thermo Fisher Scientific, Waltham, MA, USA) were used to determine transfection efficiency. After 36 h, cells were harvested for protein detection or RNA isolation. Cells were transfected with viral miRNA mimics (Qiagen, Gaithsburg, MD, USA) at a final concentration of 15 nM for 36 h. Controls consisted of mock transfected and control mimic (Qiagen) transfected cultures. Levels of viral miRNAs were used to determine transfection efficiency using miScript PCR primer assays as described above.

Naqvi A.R., Shango J., Seal A., Shukla D, & Nares S. (2018). Viral miRNAs Alter Host Cell miRNA Profiles and Modulate Innate Immune Responses. Frontiers in Immunology, 9, 433.

+ Open protocol

+ Expand

Viral miRNA Transfection Optimization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Transient transfections were performed using Lipofectaine 2000 reagent (Life Technologies, San Diego, CA) according to manufacturer’s instructions. Red siGLO oligos (ThermoScientific, Waltham, MA) were used to determine transfection efficiency. After 36 hours, cells were harvested for RNA isolation. Cells were transfected with viral miRNA mimics at increasing concentrations of 10, 20 and 50 nM (to determine dose effects) for 36 hours. We noticed robust (~90%) transfection at 20 nM, therefore, we used the same concentration for most of the experiments unless specified. Controls consisted of mock transfected and control mimic (Qiagen) transfected cultures.

Naqvi A.R., Seal A., Shango J., Navarette M.B., Martinez G., Chapa G., Hasan S., Yadavalli T., Jaishankar D., Shukla D, & Nares S. (2018). Herpesvirus-encoded microRNAs detected in human gingiva alter host cell transcriptome and regulate viral infection. Biochimica et biophysica acta, 1861(5), 497-508.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!