Urea was purchased from Solaribio (Beijing, China). The L-AAs discussed in our work were obtained from Macklin (Shanghai, China), including alanine (Ala), phenylalanine (Phe), glycine (Gly), proline (Pro), lysine (Lys), cysteine (Cys), serine (Ser), histidine (His), arginine (Arg), leucine (Leu), isoleucine (Ile), valine (Val), methionine acid (Met), aspartic acid (Asp), asparagine (Asn), glutamic acid (Glu), glutamine (Gln), tryptophan (Trp), threonine (Thr), and tyrosine (Tyr). Peptides were synthesized by Anhui Guoping Pharmaceutical Co., Ltd. (Anqing, China), including alanyl-alanine (Ala-Ala), phenylalanyl-phenylalanine (Phe-Phe), and alanyl-glycyl-proline (Ala-Gly-Pro). Cell culture media DMEM and RPMI 1640 were purchased from Corning (USA).
Glutamine
Manufactured by Maclin Biochemical Technology
Sourced in China
Glutamine is a non-essential amino acid that plays a crucial role in various metabolic processes. It serves as a key source of nitrogen and energy for various cells in the body, particularly those in the immune system and the gastrointestinal tract. Glutamine is naturally produced by the body, but it can also be supplemented through dietary sources or laboratory-produced formulations.
Automatically generated - may contain errors
Lab products found in correlation
Phenylalanine, by Maclin Biochemical Technology (1 mentions)
Leucine, by Maclin Biochemical Technology (1 mentions)
Glutamic acid, by Maclin Biochemical Technology (1 mentions)
Tryptophan, by Maclin Biochemical Technology (1 mentions)
Glycine, by Maclin Biochemical Technology (1 mentions)
Dulbecco modified eagle medium (dmem), by Corning (1 mentions)
Rpmi 1640, by Corning (1 mentions)
2 protocols using glutamine
1
Amino Acid and Peptide Materials for Cell Culture
2
Stem Cell Sphere Formation Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Next, 0.5% N2 and 1% B27 (Gibco, USA), bFGF and EGF (50 ng/mL, Aladdin, China), and 1% glutamine (Macklin, China) were mixed with the cells (1 × 103 cells per well) and then inoculated into ultralow adhesion plates containing FBS-free DMEM/F12. Cells were cultivated in 37 °C incubation with 5% CO2 for 15 days.
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