RNA extraction was performed using a semi-automated method with magnetic silica beads (supplied by bioMerieux, Marcy l’Etoile, France). After an incubation step at room temperature for 20 min, 100 μL of magnetic silica beads were added, and, after a further 10 min incubation, an automated procedure was performed using a nucleic acid purification system (Auto-Pure96, All Sheng Instruments, Zhejiang, China) or a Nuclisens MiniMag system (bioMerieux, Marcy l’Etoile, France). The extracted nucleic acids were then purified from potential PCR inhibitors using the OneStep PCR Inhibitor Removal Kit (Zymo Research, CA, USA).
Magnetic silica beads
Manufactured by bioMérieux
Sourced in France
Magnetic silica beads are a type of laboratory equipment used for the extraction and purification of nucleic acids, such as DNA and RNA, from various biological samples. These beads are composed of a silica matrix with a magnetic core, allowing for efficient separation and isolation of target biomolecules using a magnetic field.
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Lab products found in correlation
2 protocols using magnetic silica beads
1
Automated RNA Extraction Using Magnetic Beads
2
Automated Viral RNA Extraction and Purification
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Viral RNA extraction was carried out using a semi-automated extraction method with buffer lysis and magnetic silica. In detail, the lysis phase was performed using 10 mL of lysis buffer (NucliSENS, bioMerieux, Marcy l'Etoile, France) with 5 mL of a concentrated sample, after an incubation of 20 min at room temperature, 100 μL of magnetic silica beads (bioMerieux, Marcy l'Etoile, France) were added and, after further incubation of 10 min, an automated procedure was performed by nucleic acid purification system (Auto-Pure96, All Sheng Instruments, Zhejiang, China). Before molecular tests, the extracted nucleic acids were purified from potential PCR inhibitors using the OneStep PCR Inhibitor Removal Kit (Zymo Research, CA, USA).
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