Sciex 5500 qtrap mass spectrometer system
The Sciex 5500 QTRAP® mass spectrometer system is a high-performance analytical instrument designed for advanced quantitative and qualitative analysis. It features a hybrid quadrupole linear ion trap mass analyzer that provides enhanced sensitivity, selectivity, and data acquisition capabilities.
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4 protocols using sciex 5500 qtrap mass spectrometer system
Metabolite Identification and Quantification
Quantitative Analysis of Compounds using UFLC-QTRAP-MS/MS
Mass spectrometry detection was conducted using the electrospray ionization (ESI) source in positive mode. Curtain gas (CUR), nebulizer gas (GS1), and auxiliary gas (GS2) were set at 35, 50, and 50 psi, respectively. The ion spray voltage (IS) was set to 5500 V, and the source temperature was maintained at 550 °C. Multiple reaction monitoring (MRM) mode was employed for quantitation, with a dwell time of 50 ms for each MRM transition. Applied Biosystems Analyst software (version 1.6) was used for controlling the UFLC-QTRAP-MS/MS system, as well as for data acquisition and processing.
Quantitative Analysis of Compounds using UFLC-QTRAP-MS/MS
Mass spectrometry detection was conducted using the electrospray ionization (ESI) source in positive mode. Curtain gas (CUR), nebulizer gas (GS1), and auxiliary gas (GS2) were set at 35, 50, and 50 psi, respectively. The ion spray voltage (IS) was set to 5500 V, and the source temperature was maintained at 550 °C. Multiple reaction monitoring (MRM) mode was employed for quantitation, with a dwell time of 50 ms for each MRM transition. Applied Biosystems Analyst software (version 1.6) was used for controlling the UFLC-QTRAP-MS/MS system, as well as for data acquisition and processing.
Metabolite Identification and Quantification
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