The lipophilic PRE measurements were performed using a 1 mM (15N, 85% 2H)-labeled p7(5a) reconstituted in bicelles with q = 0.6. A stock solution of lipophilic paramagnetic agent 16-DSA (Sigma-Aldrich) was prepared at 24 mM concentration in the same buffer as that of the p7(5a) sample to prevent changes in the bicelle q value upon addition of the titrant. The progress of the titration was monitored by measuring 2D TROSY-HSQC spectrum at each of the following 16-DSA concentrations: 0, 0.25, 0.5, 1, 1.5, 2, 4, and 8 mM. The residue-specific PREamp was determined by fitting the peak intensity decay as a function of [16-DSA] to Eq. 3 .
16 dsa
Manufactured by Merck Group
Sourced in United States
16-DSA is a laboratory reagent used for biochemical and biophysical analysis. It is a fatty acid derivative that serves as a spin-label probe for membrane and protein structure studies. The core function of 16-DSA is to provide a stable and well-characterized nitroxide group that can be utilized in various experimental techniques, such as electron paramagnetic resonance (EPR) spectroscopy.
Automatically generated - may contain errors
Lab products found in correlation
5 dsa, by Merck Group (2 mentions)
Geraniol, by Merck Group (1 mentions)
Cholesterol chol, by Merck Group (1 mentions)
Sodium fluorescein nafl, by Merck Group (1 mentions)
D limonene, by Merck Group (1 mentions)
Lissamine rhodamine b 1 2 dihexadecanoyl sn glycero 3 phosphoethanolamine triethylammonium salt rh pe, by Thermo Fisher Scientific (1 mentions)
Tween 20, by Thermo Fisher Scientific (1 mentions)
1 8 cineole, by Merck Group (1 mentions)
Hemin, by Merck Group (1 mentions)
4 4 dimethyl 4 silapentane 1 sulfonic acid dss, by Cambridge Isotopes (1 mentions)
Sodium dithionite, by Merck Group (1 mentions)
2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid (abts), by Merck Group (1 mentions)
Cytochrome c, by Merck Group (1 mentions)
Mitoxantrone dihydrochloride, by Merck Group (1 mentions)
Natdc, by Merck Group (1 mentions)
4 protocols using 16 dsa
1
Lipophilic Paramagnetic Titration of p7(5a)
2
Lipid Bilayer Preparation and Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Non-hydrogenated egg phosphatidylcholine (PC) (Coatsome NC-50; PC purity≥95%) was purchased from NOF Corporation (Tokyo, Japan). Cholesterol (Chol) was purchased from Sigma-Aldrich, St Louis, MO, USA. Tween 20 was purchased from Ajax Finechem (Auckland, New Zealand). Sodium fluorescein (NaFl), d-limonene, 1,8-cineole, and geraniol were purchased from Sigma-Aldrich. 5-DSA and 16-DSA were purchased from Sigma-Aldrich. Lissamine™ rhodamine B 1, 2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine triethylammonium salt (Rh-PE) was purchased from Invitrogen, CA, USA. All other reagents were of analytical grade and were commercially available.
3
Peptide Purification and Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Peptides were commercially synthesized from GL-Biochem (Shanghai, China). Crude peptides were subjected to purification by reverse phase HPLC Waters™ using a C4 column (300 Å pore size, 5 μM particle size). A linear gradient of acetonitrile/water (both solutions containing 0.1% v/v TFA) was used to elute the peptides while maintaining a constant flow rate of 2 mL min–1. The major sharp peak fraction obtained was then pooled and lyophilized. The masses of the peptides were confirmed by mass spectrometry. DPC and deuterated compounds (DPC-d38, D2O) were purchased from Avanti polar lipids (Alabama, USA) and Cambridge Isotope Laboratories Inc. (Massachusetts, USA), respectively. 16-DSA was obtained from Sigma (St. Louis, MO, USA). 4,4-Dimethyl-4-silapentane-1-sulfonic acid (DSS) was acquired from Cambridge Isotope Laboratories Inc. (Massachusetts, USA). Other chemicals including sodium dithionite, hemin, ABTS, and cytochrome c were of analytical grade and purchased from Sigma-Aldrich.
4
Mitoxantrone-based Liposome Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mitoxantrone dihydrochloride, NaTC, NaTDC and the spin probes 5-DSA, 12-DSA and 16-DSA were analytical grade and supplied by Sigma Aldrich (St. Louis, MO, USA). All the compounds were used without further purification. Experiments were performed in 0.1 M phosphate buffer (pH 7.4) and 0.1 M carbonate buffer (pH 10) and deionized water (18.2 MΩcm, Mili-Q water purification system) was used for the preparation of solutions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!