Rabbit anti cytokeratin 7 polyclonal antibody

Manufactured by Novus Biologicals

Sourced in United Kingdom

Rabbit-Anti-Cytokeratin 7 polyclonal antibody is a laboratory reagent used to detect the presence of the cytokeratin 7 protein in biological samples. It is produced in rabbits and reacts specifically with the cytokeratin 7 antigen.

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Lab products found in correlation

Goat anti vimentin polyclonal antibody, by Santa Cruz Biotechnology (1 mentions) Vectashield with dapi, by Vector Laboratories (1 mentions) Rhodamine rx, by Jackson ImmunoResearch (1 mentions)

2 protocols using rabbit anti cytokeratin 7 polyclonal antibody

Immunohistochemical Staining Protocol

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Detailed procedures for IHC staining have been described previously^{39 (link)}. Signals were visualized using SuperPicTure™ Polymer Detection kits (Life Technologies). The antibodies used were: Rabbit-Anti-Cytokeratin 7 polyclonal antibody (Novus); Rat-Anti-Cytokeratin 8 monoclonal antibody (DSHB); Goat-Anti-Vimentin polyclonal antibody (Santa Cruz).

Yen C.H., Lai C.C., Liao C.C., Wang S.F., Liao Y.J., Tung C.Y., Hung J.H., Huang S.F, & Chen Y.M. (2017). Characterization of a new murine cell line of sarcomatoid hepatocellular carcinoma and its application for biomarker/therapy development. Scientific Reports, 7, 3052.

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Decidual Immune Cell Characterization

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Sections of control and infected decidua were immunostained for immunofluorescence using standard methods and mounted in VectaShield with DAPI (Vector Laboratories, Burlingame, CA). Antibodies used include monoclonal antibody CH443 targeting HCMV IE, produced in the Pereira laboratory, and the following antibodies obtained from commercial sources: mouse and rabbit anti-CD3 mAbs (Abeam, Cambridge, United Kingdom), rabbit anti-CD4 mAb (Abeam, Cambridge, United Kingdom), rat anti-CD4 mAb (BioRad Laboratories, Hercules, CA), mouse anti-CD8 mAb (BD Pharmingen, San Diego, CA), rabbit anti-IFN-γ polyclonal antibody (Abcam, Cambridge, MA) mouse anti-granulysin mAb (Santa Cruz Biotechnology, Dallas, TX), rabbit and mouse anti-CD32A (Abeam, Cambridge, United Kingdom), goat anti-CD56 polyclonal antibody (R&D Systems, Minneapolis, MN), rabbit anti-CD163 polyclonal antibody (Abeam, Cambridge, United Kingdom), rabbit anti-CD209 (DC-SIGN) polyclonal antibody (Abeam, Cambridge, United Kingdom), rabbit anti-cytokeratin 7 polyclonal antibody (Novus Biologicals, Centennial, CO), mouse anti-cytokeratin 7 mAb (Dako Products, Santa Clara, CA), rabbit anti-vimentin mAb (Abeam, Cambridge, United Kingdom), mouse anti-vimentin IgM mAb (Sigma-Aldrich, St. Louis, MO). Fluorescently labeled secondary antibodies (FITC, Rhodamine RX, and Cy5) were obtained from Jackson Immunoresearch (West Grove, PA).

Tabata T., Petitt M., Fang-Hoover J, & Pereira L. (2019). Survey of cellular immune responses to HCMV infection in the microenvironment of the uterine-placental interface. Medical microbiology and immunology, 208(3-4), 475-485.

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