The immunohistochemistry (IHC) staining reaction was performed on FFPE tissue samples. IHC was performed on the Ventana BenchMark ULTRA IHC/ISH autostaining system using primary antibodies anti-CD8 (Ventana anti-CD8, clone SP57, rabbit monoclonal primary antibody) and anti-CD56 (Cell Marque, clone MRQ-42, rabbit monoclonal antibody) after antigen retrieval in Cell Conditioning 1 buffer followed by detection with the Ultra View HRP system (Roche/Ventana).
Ultra view hrp system
Manufactured by Roche
The Ultra View HRP system is a laboratory equipment designed for immunohistochemistry (IHC) analysis. It provides automated slide processing and detection for IHC staining. The system utilizes a horseradish peroxidase (HRP)-based detection method to visualize target proteins in tissue samples.
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Lab products found in correlation
2 protocols using ultra view hrp system
1
Immunohistochemistry of CD8 and CD56
2
Immunohistochemical Profiling of Brain Tumors
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Immunohistochemical reactions were performed on 4 μm thick FFPE sections using the Ventana BenchMark ULTRA IHC/ISH autostaining system. The following Ventana primary antibodies were applied: for Glial Fibrillary Acidic Protein (GFAP), anti-GFAP [clone EP672Y], Rabbit Monoclonal Antibody; for Ki-67, anti-Ki-67 [clone 30–9], Rabbit Monoclonal Antibody, for Olig2, anti-Olig2 [clone EP112], Rabbit Monoclonal Antibody and for Synaptophysin, anti-Synaptophysin [clone MRQ-40], Rabbit Monoclonal Antibody. Antigen retrieval was performed in CC1 buffer followed by detection with the Ultra View HRP system (Roche/Ventana).
Expression of BCOR was detected using commercially available antibody (sc-514,576; Santa Cruz, Dallas, TX), [clone C10] at a dilution of 1:400. Antigen retrieval was performed using Target Retrieval Solution, High pH, (DAKO, Glostrup, Denmark) for 30 min in 99.5 °C. The specificity of BCOR immunoexpression was tested in classic MB and normal testis. Whole slides were scanned in Hamamatsu NanoZoomer 2.0 RS scanner (Hamamatsu Photonics, Hamamatsu, Japan) at the original magnification 40 × .
Expression of BCOR was detected using commercially available antibody (sc-514,576; Santa Cruz, Dallas, TX), [clone C10] at a dilution of 1:400. Antigen retrieval was performed using Target Retrieval Solution, High pH, (DAKO, Glostrup, Denmark) for 30 min in 99.5 °C. The specificity of BCOR immunoexpression was tested in classic MB and normal testis. Whole slides were scanned in Hamamatsu NanoZoomer 2.0 RS scanner (Hamamatsu Photonics, Hamamatsu, Japan) at the original magnification 40 × .
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