Anti flag and actin antibodies

Manufactured by Merck Group

Sourced in United States

Anti-Flag and actin antibodies are laboratory reagents used for protein detection and analysis. The Anti-Flag antibody recognizes a specific epitope tag, allowing identification and isolation of tagged proteins. The actin antibody detects the actin cytoskeletal protein, which is commonly used as a loading control. These antibodies provide tools for researchers to study protein expression and interactions.

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Lab products found in correlation

Anti ha, by Cell Signaling Technology (1 mentions) Anti phosphohistone γh2ax ser139, by Merck Group (1 mentions) Anti pcdk9, by Cell Signaling Technology (1 mentions) Fluorescent dye conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Anti plk1, by Cell Signaling Technology (1 mentions)

2 protocols using anti flag and actin antibodies

Antibody Source and Characterization

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Antibodies were purchased from the following sources: anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and Myc antibodies were from Santa Cruz Biotechnology (USA); anti-Flag and actin antibodies were from Sigma-Aldrich; goat anti-TEN1 antibody was from Santa Cruz Biotechnology. HCV core, NS3, and NS5A antibodies have been described elsewhere (Lim et al., 2011 (link)). Rabbit anti-TEN1 antibody was kindly provided by Professor Fuyuki Ishikawa (Kyoto University, Japan).

Lim Y.S., Nguyen M.T., Pham T.X., Huynh T.T., Park E.M., Choi D.H., Kang S.M., Tark D, & Hwang S.B. (2021). Hepatitis C Virus Nonstructural 5A Protein Interacts with Telomere Length Regulation Protein: Implications for Telomere Shortening in Patients Infected with HCV. Molecules and Cells, 45(3), 148-157.

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Antibody reagents for DNA damage signaling

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Anti–phospho-Histone γ-H2AX (Ser139) was from Millipore (Billerica, MA); MDC1 and 53BP1 antibodies were gifts from Dr. Zhenkun Lou, Mayo Clinic. Anti-Bora was obtained from New England Peptide (Gardner, MA). Anti–HA, GST, anti-PLK1 as well as anti-pCDK9 and CDK9 were from Cell Signaling Technology, Inc (Danvers, MA); anti–FLAG and actin antibodies were purchased from Sigma-Aldrich, Inc. (St. Louis, MO); and the horseradish peroxidase–conjugated secondary antibodies against mouse and rabbit were obtained from Santa Cruz Biotechnology, Inc (Santa Cruz, CA). Fluorescent dye–conjugated secondary antibodies were obtained from Invitrogen Corp (Carlsbad, CA).

Cairns J., Peng Y., Yee V.C., Lou Z, & Wang L. (2015). Bora Downregulation Results in Radioresistance by Promoting Repair of Double Strand Breaks. PLoS ONE, 10(3), e0119208.

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