Jem 1010

Manufactured by Thermo Fisher Scientific

The JEM-1010 is a transmission electron microscope (TEM) designed and manufactured by Thermo Fisher Scientific. It is a compact and cost-effective TEM instrument that provides high-resolution imaging capabilities for a variety of sample types.

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Lab products found in correlation

Uranyl acetate and lead citrate, by Electron Microscopy Sciences (1 mentions) Sodium cacodylate trihydrate, by Merck Group (1 mentions) Osmium tetroxide solution, by Merck Group (1 mentions) Glutaraldehyde, by Electron Microscopy Sciences (1 mentions) Has 3000, by Malvern Panalytical (1 mentions) V 530, by Jasco (1 mentions)

2 protocols using jem 1010

Ultrastructural Analysis of Kidney Organoids

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Kidney organoid cultures were fixed in PBS + 4 % paraformaldehyde for five minutes, collected by scraping, pelleted at 300 g, and resuspended in EM fix: 0.15 M sodium cacodylate trihydrate (Sigma) dissolved in water (pH 7.3) containing 4% formaldehyde and 2% glutaraldehyde (Electron Microscopy Sciences). Kidney tissues (1 mm diameter) were placed directly into EM fix. Samples were post-fixed with osmium tetroxide solution (Sigma), dehydrated in serial ethanol dilutions (Sigma), and embedded in epoxy resin. Ultrathin sections (80 nm) were mounted on 200 mesh copper grids, stained with uranyl acetate and lead citrate (Electron Microscopy Sciences), and imaged with JEOL JEM-1010 and FEI Tecnai G2 Spirit TEMs. Images were representative of at least two kidneys containing numerous glomeruli per condition.

Kim Y.K., Refaeli I., Brooks C.R., Jing P., Gulieva R.E., Hughes M.R., Cruz N.M., Liu Y., Churchill A.J., Wang Y., Fu H., Pippin J.W., Lin L.Y., Shankland S.J., Vogl A.W., McNagny K.M, & Freedman B.S. (2017). Gene-edited human kidney organoids reveal mechanisms of disease in podocyte development. Stem cells (Dayton, Ohio), 35(12), 2366-2378.

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Nanoparticle Characterization via Spectroscopy

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The LSPR absorption of the nanoparticles were measured at 400 to 900-nm wavelengths by UV-vis spectrophotometry (JASCO, V-530). Transmission electron microscopy (TEM, JEOL JEM 1010, FEI, Tecnai) was used for measuring the size and crystallographic structure of the nanoparticles. Dynamic light scattering (DLS) of the synthesized nanoparticles were measured by a ζ-sizer (HAs 3000, MALVERN, USA). The characterization of the nanoparticles are reported in

Table S1

and

Figure S1

Figure S4

section of the supplementary data section.
The nanostructures were characterized by a dynamic light scattering (DLS) (ζ-sizer) device to identify the size distribution and charge evaluation of the nanoparticles; see the

Figure S1

section of the supplementary data section.

Nasseri B., Turk M., Kosemehmetoglu K., Kaya M., Piskin E., Rabiee N, & Webster T.J. (2020). The Pimpled Gold Nanosphere: A Superior Candidate for Plasmonic Photothermal Therapy. International Journal of Nanomedicine, 15, 2903-2920.

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