2,4-Dinitrophenylhyrazine (DNPH), reduced glutathione (GSH), oxidized glutathione (GSSG), N-ethylmaleimide (NEM), N-(1-naphthyl) ethylenediamine dihydrochloride, 5,5'-dithiobis-2-nitrobenzoic acid (DTNB), metaphosphoric acid, NADPH, nitroblue tetrazolium salt (NBT), and O-phthaldehyde (OPT) were purchased from Sigma-Aldrich, Chemie GmbH, Germany. Bradford reagent and BSA as a protein standard were obtained from Bio-Rad Laboratories, Hercules, CA, USA. Cyperforce (Cypermethrin) was a product of Ghada Chemical Ltd, China and Masters (λ-cyhalothrin) was purchased from Sinochem Ningbo Ltd, China. All other chemicals were of analytical grade. Absolute ethanol was used as a solvent in the preparation of the insecticides and bio-insecticides samples.
Nitroblue tetrazolium salt
Manufactured by Merck Group
Sourced in United States
Nitroblue tetrazolium salt is a chemical compound used in various laboratory techniques. It is commonly employed as an electron acceptor in histochemical and cytochemical assays to detect the presence of specific enzymes or metabolic activities within cells or tissue samples. The core function of nitroblue tetrazolium salt is to serve as a colorimetric indicator, undergoing reduction to produce a dark-colored, insoluble formazan product that can be visualized and quantified.
Automatically generated - may contain errors
Lab products found in correlation
N 1 naphthyl ethylenediamine dihydrochloride, by Merck Group (1 mentions)
Reduced glutathione, by Merck Group (1 mentions)
Oxidized glutathione, by Merck Group (1 mentions)
Bradford reagent, by Bio-Rad (1 mentions)
Bovine serum albumin (bsa), by Bio-Rad (1 mentions)
Nadph, by Merck Group (1 mentions)
N ethylmaleimide, by Merck Group (1 mentions)
O phthaldehyde, by Merck Group (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Caffeic acid, by Merck Group (1 mentions)
Linoleic acid, by Merck Group (1 mentions)
Ursolic acid, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Trolox, by Merck Group (1 mentions)
Alcl3, by Merck Group (1 mentions)
Nimesulide, by Merck Group (1 mentions)
Neocuproin, by Merck Group (1 mentions)
3 5 dinitrosalicylic acid, by Merck Group (1 mentions)
L3126, by Merck Group (1 mentions)
Barnsted easypure water filter, by Thermo Fisher Scientific (1 mentions)
5 protocols using nitroblue tetrazolium salt
1
Insecticide Assay Protocol Using Chemical Reagents
2
Enzyme Inhibition and Antioxidant Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA): AlCl3, amberlite XAD7 HP, α-amylase from porcine pancreas (A3176; EC 3.2.1.1), α-glucosidase from Saccharomyces cerevisiae (G5003; EC 3.2.1.20), DPPH (2,2-diphenyl-1-picrylhydrazyl radical), DMAC (4-[dimethylamino]cinnamaldehyde), 3,5-dinitrosalicylic acid, catechin, caffeic acid, CuCl2, hypoxanthine, indomethacin, linoleic acid (L-5900), lipase from porcine pancreas type II (L-3126; EC 3.1.1.3), Na2CO3, nimesulide, 4-nitrophenyl-α-d -glucopyranoside, nitro blue tetrazolium salt, p-nitrophenyl palmitate, sodium acetate, soy lipooxygenase-15 (L7395; EC 1.13.11.12), starch, 2,4,6-tri(2-pyridyl)1,3,5-triazine (TPTZ), triton X-100, tris, and ursolic acid. From Merck (Darmstadt, Germany): Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), FeCl3∙6H2O, neocuproin, potassium sodium tartrate and HPLC-grade methanol. Ammonium acetate was from JT Baker (Xalostoc, Mexico). The sodium and potassium phosphate salts were from Scharlau Chemicals (Barcelona, Spain). Orlistat was from Laboratorio Chile (Santiago, Chile). Culture medium, antibiotics and fetal bovine serum were obtained from Invitrogen Corp. (Waltham, MA, USA). Ultrapure water was obtained using a Barnsted EasyPure water filter (Thermo Scientific, Marietta, OH, USA).
3
Synthesis and Evaluation of Bioactive Compounds
4
Optimizing G6PDH Assay Conditions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Example 2
G6PDH was purchased from Calzyme Laboratories EC 1.1.1.49. Glucose-6-phosphate (G-6-P) was also purchased from Calzyme Laboratories, part number 046M0000. Diaphorase-1 was purchased from Nipro Medical Corporation. NADP+ was purchased from Calzyme Laboratories, part number 218J0000. Nitro Blue Tetrazolium (NBT) salt was purchased from Sigma-Aldrich, part number N6875. Several iterations were carried out to test the immobilized concentrations of G-6-P, as well as with and without G-6-P, on the secondary Membrane 2B. In addition, several iterations were carried out to test the immobilized concentrations of diaphorase, NADP+, and tetrazolium salt on a thin layer film format, reagent Membrane 3B, of the test strips. Both Membrane 2B and 3B were coated and dried at 55° C. for 20 minutes.
The tetrazolium chosen for the experiment was nitro blue tetrazolium chloride (NBT). NBT was dissolved in a methanol solution with polyvinyl alcohol. The rate of change in percent reflectance (% R) was measured at 565 nm to determine the optimal concentrations of all immobilized bioactive components. The samples were altered for both Hgb and spiked G6PDH plasma, encompassing the clinically significant range for both anemia and G6PDH deficiency. Percent reflectance (% R) was measured using a spectrophotometer. The data reveals excellent agreement with both G6PDH and Hgb dose response curves.
5
Analytical Techniques for Natural Product Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Column chromatography was carried out using silica gel 60 (70-230 mesh ASTM). Analytical thin-layer chromatography (TLC) was performed on silica gel 60 F254 aluminum sheets. 1H- and 13C-NMR spectra were recorded on a Bruker AVANCE 300 NMR spectrometer. FTIR spectra were obtained using a universal attenuated total reflectance attached on a Perkin-Elmer Spectrum One spectrometer. High resolution mass spectra (HRMS) were recorded on a Bruker Daltonics (microTOF). Melting points were determined using a Griffin melting point apparatus and were uncorrected. Chemicals and reagents were purchased: vitamin E, DPPH (2,2-diphenyl-1-picrylhydrazyl), nitro blue tetrazolium (NBT) salt, L-methionine, riboflavin, Triton-100 and superoxide dismutase (SOD, bovine erythrocytes) from Sigma, USA. DMSO (dimethyl sulfoxide, 99.9 %) from RCI Labscan, Thailand; methanol from Merck, Germany; ampicillin, ciprofloxacin and tetracycline from Sigma, USA; Muller Hinton Broth and Muller Hinton Agar from Becton Dickinson, USA; and sodium chloride from Merck, German. Solvents were analytical grades.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!