Cetyltrimethylammonium bromide (CTAB, >98.0%), cetyltrimethylammonium chloride (CTAC, 25% water solution), hydroquinone (HQ, 99%), L-ascorbic acid (AA, >99.9%), 4-nitrobenzenethiol (NBT), Hydrogen peroxide (37%), and sodium borohydride (NaBH4, 99%) were purchased from Sigma-Aldrich. Hydrogen tetrachloroaurate trihydrate (HAuCl4·3H2O) and silver nitrate (AgNO3, >99%) were purchased from Alfa Aesar. Ultrapure water obtained from a Milli-Q Integral 5 system was used in all experiments.
Cetyltrimethylammonium chloride
Manufactured by Merck Group
Sourced in United States
Cetyltrimethylammonium chloride is a cationic surfactant commonly used as a laboratory reagent. It is a white, crystalline solid with the chemical formula CH3(CH2)15N(CH3)3Cl. The primary function of cetyltrimethylammonium chloride is to act as a detergent, emulsifier, and phase transfer agent in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Cetyltrimethylammonium bromide, by Merck Group (16 mentions)
Sodium borohydride, by Merck Group (15 mentions)
L ascorbic acid, by Merck Group (11 mentions)
Silver nitrate, by Merck Group (8 mentions)
Hydrochloric acid (hcl), by Merck Group (5 mentions)
Polyvinylpyrrolidone, by Merck Group (4 mentions)
Sodium hydroxide (naoh), by Merck Group (4 mentions)
Tetraethyl orthosilicate, by Merck Group (4 mentions)
Citric acid, by Merck Group (4 mentions)
Gold 3 chloride trihydrate, by Merck Group (4 mentions)
Ascorbic acid, by Merck Group (4 mentions)
Benzyldimethylhexadecylammonium chloride, by Merck Group (3 mentions)
Cyclohexane, by Xilong (3 mentions)
Hydroquinone, by Merck Group (2 mentions)
Hydrogen peroxide, by Merck Group (2 mentions)
Tetraethyl orthosilicate, by Thermo Fisher Scientific (2 mentions)
Ethanol, by Merck Group (2 mentions)
Acetonitrile, by Merck Group (2 mentions)
Bismuth 3 nitrate pentahydrate bi no3 3 5h2o, by Merck Group (2 mentions)
Sodium citrate, by Merck Group (2 mentions)
41 protocols using cetyltrimethylammonium chloride
1
Synthesis of Gold and Silver Nanoparticles
2
Lipid-based Nanoparticle Synthesis Protocol
3
Synthesis and Characterization of SNP Variants
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This study examined three types of SNPs: spherical (S-SNPs), mesoporous (M-SNPs) and PEGylated (P-SNPs; PEG, polyethylene glycol). To prepare the S-SNPs, 11.87 ml of ethanol (Sigma-Aldrich, St. Louis, MO, USA), 2.1 ml of distilled (DI) water, 0.67 ml of tetraethoxysilane (Sigma-Aldrich) and 0.36 ml of 5 M NH4OH (Sigma-Aldrich) were mixed in a vial and stirred for 24 h.
M-SNPs were synthesized using 53.4 g of DI water, 6.24 g of cetyltrimethylammonium chloride (Sigma-Aldrich) and 0.3 g of sodium acetate (Sigma-Aldrich), were mixed well, and then heated in a 65 °C oil bath. After heating for 1 h, 3.9 ml of tetraethoxysilane was slowly added to the mixture and then stirred for 24 h. The resulting suspensions were purified by centrifugation, re-dispersed in ethanol and DI water, and then finally re-dispersed in DI water.
P-SNPs were prepared by surface functionalization of S-SNP. Briefly, thiol-functionalized S-SNP was prepared by adding (3-mercaptopropyl) trimethoxysilane (10% of tetraethoxysilane) 5 h after the addition of NH4OH during synthesis of S-SNP. After purification, these particles were conjugated with maleimide-functionalized PEG (molecular weight: 2000) in DI water for 24 h (weight ratio: ~0.5:1). Excess amount of PEG was removed by centrifugation and dispersed in DI water to obtain P-SNP.
M-SNPs were synthesized using 53.4 g of DI water, 6.24 g of cetyltrimethylammonium chloride (Sigma-Aldrich) and 0.3 g of sodium acetate (Sigma-Aldrich), were mixed well, and then heated in a 65 °C oil bath. After heating for 1 h, 3.9 ml of tetraethoxysilane was slowly added to the mixture and then stirred for 24 h. The resulting suspensions were purified by centrifugation, re-dispersed in ethanol and DI water, and then finally re-dispersed in DI water.
P-SNPs were prepared by surface functionalization of S-SNP. Briefly, thiol-functionalized S-SNP was prepared by adding (3-mercaptopropyl) trimethoxysilane (10% of tetraethoxysilane) 5 h after the addition of NH4OH during synthesis of S-SNP. After purification, these particles were conjugated with maleimide-functionalized PEG (molecular weight: 2000) in DI water for 24 h (weight ratio: ~0.5:1). Excess amount of PEG was removed by centrifugation and dispersed in DI water to obtain P-SNP.
4
Colorimetric Biosensing of SARS-CoV-2 Antigens
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Silver nitrate (AgNO3), sodium citrate, cetyltrimethylammonium bromide (CTAB), ascorbic acid (AA), dichloromethane, n-hexane, polyvinylpyrrolidone (PVP) and 4-mercaptobenzoic acid (4-MBA) were all bought from Aladdin Reagent Co., Ltd. Gold(III) chloride trihydrate (HAuCl4·3H2O), sodium borohydride, 11-Mercaptoundecanoic acid (MUA), bovine serum albumin (BSA), polyvinyl pyrrolidone (PVP) and cetyltrimethylammonium chloride (CTAC) were all obtained from Sigma Aldrich. Phosphate-buffered saline (PBS), 1 × TBST/Tween 20 buffer were prepared in-house. SARS-CoV-2 spike antigen protein, human immunodeficiency virus type 1 p24 antigen (HIV-1 p24), Epstein-Barr virus glycoprotein 350 (EBV GP350), carcinoembryonic antigen (CEA), alpha fetoprotein (AFP), SARS-CoV-2 spike antibody were purchased from Beijing Yiqiao Shenzhou Science and Technology Co., Ltd. The clinical saliva samples, clinical serum samples and whole blood were obtained from The First Hospital of Jilin University. The deionized water with the resistivity of 18.2 MΩ was used in all experiments.
5
Synthesis and Characterization of Nanomaterials
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Ammonium hydroxide (28–30%), tetraethyl orthosilicate (TEOS, 96%), ethanol absolute (99.7%), silver nitrate (99.5%), sodium chloride (99.5%), potassium chloride (99.5%), and trifluoroacetic acid (TFA, 99%) were purchased from Sinopharm Chemical Reagent Beijing Co., Ltd (Beijing, China). Acetonitrile (ACN, 99%), α-cyano-4-hydroxycinnamic acid (CHCA, 99%), dithiothreitol (DTT, 99%), cetyltrimethylammonium chloride (CTAC, 99%), albumin from bovine serum (BSA, 98%), polyvinylpyrrolidone (PVP, MW = 40,000), D -glucose (99.5%), L -methionine (99%), D -mannitol (99%), L -lactic acid (98%), L -arginine (99.5%), L -tryptophan (98%), uric acid (99%), and DL -phenylalanine (99%) were purchased from Sigma, USA. Tris base and phosphate-buffered saline (PBS, 10×, pH 7.4, cell-culture grade) were purchased from Yeason Biotechnology Co., Ltd (Shanghai, China). The isotopic internal standards of glucose (labeled with six 13C or one 13C) and mannitol (labeled with one 13C) were purchased from Cambridge Isotope Laboratories (CIL, USA). Thiol-aptamers were obtained from Sangon Biotech Co., Ltd. (Shanghai, China). All aqueous solutions were prepared using deionized water (18.2 MΩ cm, Milli-Q, Millipore, GmbH) throughout the experiments.
6
Synthesis and Functionalization of Gold Nanoparticles
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Gold chloride trihydrate (HAuCl4·3H2O, 99.9%), cetyltrimethylammonium chloride (CTAC, 98%), trisodium citrate (TSC, 99%), sodium dodecyl sulfate (SDS, 99%), sodium borohydride (NaBH4, 98%), l -ascorbic acid (AA, 99%), hydrochloric acid (HCl, 5 M), aniline (99%), potassium persulfate (KPS, 99%), hydrogen peroxide (H2O2, in water, 30 wt%), sodium hydroxide (NaOH, 99%), l -cysteine (l -Cys, 99%), d -cysteine (d -Cys, 99%), and l -glutathione (l -GSH, 98%,) were purchased from Sigma-Aldrich. Cetyltrimethylammonium bromide (CTAB, 98%) was obtained from Alfa Aesar. Tetraethyl orthosilicate (TEOS, 98%) was purchased from Acros Organics. Rhodamine 640 perchlorate (R640) was purchased from Exciton-Luxottica. d -glutathione (d -GSH, 99%) was purchased from Apeptide. Potassium iodide (KI, 99%) was purchased from Aladdin Reagent. Deionized water with a resistivity of 18.2 MΩ cm and produced by a Direct-Q 5 ultraviolet water purification system was used throughout all experiments.
7
Synthesis and Characterization of Nanomaterials
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Aluminum tri-sec-butoxide (ASB) Al[OCH(CH3)C2H5]3, bismuth(III) nitrate
pentahydrate (Bi(NO3)3·5H2O),
cetyltrimethylammonium bromide (CTAB,
≥99%), cetyltrimethylammonium chloride (CTAC, 25 wt % in water),
and glacial acetic acid were purchased from Sigma-Aldrich. Seven TrOPs
were selected for this study, based on their physicochemical properties
(Supporting Information Table S1).15 (link) All standards (purity > 99%) were obtained
from
Sigma-Aldrich, except for IPRM and IPMD (European Pharmacopeia reference
standards, France). Stock solutions were prepared by dissolving in
deionized (DI) water (Direct-Q3 UV system, Millipore-France). LC-grade
methanol and water were purchased from Bio-Lab (Jerusalem, Israel).
pentahydrate (Bi(NO3)3·5H2O),
cetyltrimethylammonium bromide (CTAB,
≥99%), cetyltrimethylammonium chloride (CTAC, 25 wt % in water),
and glacial acetic acid were purchased from Sigma-Aldrich. Seven TrOPs
were selected for this study, based on their physicochemical properties
(
from
Sigma-Aldrich, except for IPRM and IPMD (European Pharmacopeia reference
standards, France). Stock solutions were prepared by dissolving in
deionized (DI) water (Direct-Q3 UV system, Millipore-France). LC-grade
methanol and water were purchased from Bio-Lab (Jerusalem, Israel).
8
Synthesis and Characterization of Multifunctional Luminescent Probes
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GdCl3·6H2O, EuCl3·6H2O, sodium citrate (Na3Cit), sodium fluoride (NaF), cetyltrimethylammonium chloride (CTAC), tetraethyl orthosilicate (TEOS), 3-mercaptopropyltrimethoxysilane (MPTES), 1,3-diphenylisobenzofuran (DPBF), t-butyl nitrite, indocyanine green (ICG), methylene blue (MB), and 3-coumarin-carboxylic acid (3-CCA) were purchased from Sigma-Aldrich. Reactive Oxygen Species Assay Kit (2',7'-dichlorofluorescein diacetate (DCFH-DA)), and Annexin V-FITC/PI Apoptosis Detection Kit, Nitric Oxide Detection Kit (3-amino-4-aminomethyl-2',7'-difluorescein diacetate (DAF-FM DA)) were obtained from Beyotime Biotechnology Co., Ltd, China. NH3·H2O (25%-28%), HCl (36%-38%), triethanolamine (TEA), sodium chloride (NaCl), ethanol (99.5%), absolute methanol, and toluene were purchased from Guangfu Fine Chemical Research Institute (Tianjin, China). All chemical agents were of analytical grade and used without any purification. Deionized (DI) water (18.2 MΩ cm) was obtained from a Milli-Q ultrapure water system.
9
Synthesis of Gold and Silver Nanoparticles
10
Synthesis of Silica Nanoparticles with PEG Coating
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Cetyltrimethylammonium chloride (CTAC) was purchased from Sigma-Aldrich. Hexadecyl cetyltrimethylammonium bromide (CTAB), triethanolamine (TEA), tetraethyl orthosilicate (TEOS), ethanol, ammonium hydroxide (NH3·H2O) and hydrochloric acid (HCl) were obtained from Sinopharm Chemical Reagent Co. Methoxy polyethylene glycol (PEG) silane, MW 2000 (Me-PEG2000-silane) was bought from JenKem Technology. All the mentioned chemicals and regents were used directly without further purification. Deionized water (DI water) was used in all experiments.
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