All the tonsillar specimens were fixed in 10% formol saline for 24 h, washed, then exposed to serial concentrations of ethyl alcohol, and ended with absolute alcohol (for complete dehydration). Specimens were cleared in xylene and embedded in paraffin for 24 h. The rotary microtome prepared paraffin wax tissue blocks for sectioning at 4 μm thicknesses (LEICA RM 2125 UK). The obtained tissue sections were collected on glass slides, deparaffinized, and stained with H&E11 and Sirius red12 (link). H&E stain to assess the general histological structure of palatine tonsil while Sirius red is used to assess collagen fibers deposition (fibrosis).
Slides were examined under a light microscope (Primo star, ZEISS, China). The photos were taken using (an Axiocam ERc 5 s, ZEISS, China) camera, at the Histology Department, Faculty of Medicine for Girls, Al Azhar University.
Slides were examined under a light microscope (Primo star, ZEISS, China). The photos were taken using (an Axiocam ERc 5 s, ZEISS, China) camera, at the Histology Department, Faculty of Medicine for Girls, Al Azhar University.