Primary antibodies against β actin a5441

7-KC and a leukocyte acid phosphatase (TRAP) kit were purchased from Sigma Chemical (St. Louis, MO, USA). Recombinant mouse M-CSF and RANKL were acquired from R & D Systems, Inc. (Minneapolis, MN, USA). The mmu-miR-107-5p mimic (miR-107-5p mimic), mmu-miR-107-5p inhibitor (anti-miR-107-5p), control mimic (con mimic), and control inhibitor (con inh), miScript RT kit, and miScript SYBR Green PCR kit were purchased from Qiagen (Hilden, Germany). The Lipofectamine 3000 and Lipofectamine™ RNAiMAX reagents were obtained from Invitrogen (Carlsbad, CA, USA). Primary antibodies against β-actin (A5441) were obtained from Sigma Chemical, whereas MKP1 (sc-373841), PTEN (sc-7974), and SHP1 (sc-287) antibodies were obtained from Santa Cruz Biotechnology (Santa Cru, CA, USA). Small interfering RNA (siRNA) against MKP1 (sc-35938) and scrambled siRNA (scRNA, sc-37007) were obtained from Santa Cruz Biotechnology. M-MLV reverse transcriptase, SYBR Green Real-Time PCR Master Mixes, psiCHECK2, and a dual-luciferase reporter assay system were obtained from Promega (Madison, WI, USA). The QIAzol reagent was purchased from Qiagen (Hilden, Germany).

EGCG was obtained from Ebeikar Tea & Extracts Co., Ltd. (Hangzhou, China). Melatonin, quercetin (Que), luteolin, chlorogenic acid, DEDTC, 2′, 7′-dichlorofluorescin diacetate (DCFH-DA), coumarin-3-carboxylic acid (3-CCA), bathocuproinedisulfonic acid disodium salt (BCS), neocuproine, catalase, mannitol, superoxide dismutase (SOD), histidine, and methionine were purchased from Sigma (St. Louis, Missouri, USA). Plasmid pBR322 DNA was obtained from New England Biolabs (Beijing, China). ECL Plus reagent and Polyvinylidene difluoride (PVDF) membrane were products of Bio-Rad Laboratories, Inc. (Hercules, CA, USA). The primary antibodies against γ-H2AX (sc-101696) and anti-mouse (sc-2489) secondary antibody were obtained from Santa Cruz (Dallas, TX, USA). The primary antibodies against β-actin (A5441) were purchased from Sigma. The primary antibodies against cleaved caspase 3 (9662), cleaved caspase 8 (8592), PARP (9542) and anti-rabbit (7074) secondary antibodies were products of Cell Signal Technology, Inc. (Danvers, MA, USA). The primary antibody against thrombospondin-1 (TSP-1) (ab85762) and ceruloplasmin (ab48614) were products of Abcam (Cambridge, UK). Other chemicals were of the highest grade available.