MEM-α, glutamine, HEPES, penicillin-streptomycin, trypsin-EDTA, DPBS, sodium pyruvate, and HBSS were purchased from Thermo Fisher Scientific (Waltham, MA, USA; Invitrogen brand). FBS was purchased from HyClone Laboratories, Inc. (Logan, UT, USA). Dabrafenib was purchased from LC Laboratories (Woburn, MA, USA). 3-aminopropylphosphonate (3-APP) was purchased from AmBeed, Inc. (Arlington Heights, IL, USA). Trimethylsilyl propanoic acid (TSP) was purchased from Sigma-Aldrich, Inc. (St. Louis, MO, USA).
Trimethylsilylpropanoic acid tsp
Manufactured by Merck Group
Sourced in United States, Canada, Italy
Trimethylsilylpropanoic acid (TSP) is a chemical compound commonly used as a reference compound in nuclear magnetic resonance (NMR) spectroscopy. It is a stable, water-soluble compound with a well-defined 1H NMR signal that can be used to calibrate the chemical shift scale in NMR experiments.
Automatically generated - may contain errors
Lab products found in correlation
Trypsin edta, by Thermo Fisher Scientific (3 mentions)
Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (1 mentions)
Glutamine, by Thermo Fisher Scientific (1 mentions)
Hepes, by Thermo Fisher Scientific (1 mentions)
α mem, by Thermo Fisher Scientific (1 mentions)
Hanks balanced salt solution (hbss), by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Cytiva (1 mentions)
Dabrafenib, by LC Laboratories (1 mentions)
Potassium chloride (kcl), by Thermo Fisher Scientific (1 mentions)
Silicon oil, by Merck Group (1 mentions)
Polydimethyldiallyl ammonium chloride, by Merck Group (1 mentions)
Dimethyl sulphate, by Merck Group (1 mentions)
1 4 butane sultone, by Merck Group (1 mentions)
Para hydroxybenzoic acid, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Sulfuric acid, by Merck Group (1 mentions)
Sodium nitrate, by Merck Group (1 mentions)
4 protocols using trimethylsilylpropanoic acid tsp
1
Cell Culture Reagent Acquisition
2
Softwood Kraft Lignin Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Softwood kraft lignin (KL) was supplied by FPInnovations, Thunder Bay, ON, Canada. Kaolin powder was supplied by Old Hickory Clay Company, Hickory, KY, USA. Sodium metabisulfite (Na2S2O5) (99.0%), formaldehyde (CH2O) (37.0%), polydimethyldiallyl ammonium chloride (20.0 wt.%), sodium hydroxide (97.0%), hydrochloric acid (37.0%), sodium nitrate (99.0%), dimethyl sulphate (98.0%) sulfuric acid (98.0%), D2O isotopic purity (99.8%), trimethylsilylpropanoic acid (TSP) (99.8%), para-hydroxybenzoic acid (99.0%), silicon oil, poly (ethylene oxide) and 1,4–butane sultone (99.8%) were purchased from Sigma-Aldrich (Canada). Potassium chloride (99.0%) and potassium hydroxide solution (8 M) were purchased from Fisher Scientific (Canada). Cellulose acetate dialysis membrane (molecular weight cut-off of 1000 g/mol) was obtained from Spectrum Laboratories Inc., USA.
3
NMR Analysis of Deuterated Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All chemicals used in this study were of analytical grade. Deuterium oxide (purity 99.9% in D) and the internal standard trimethylsilylpropanoic acid (TSP) for NMR analysis were purchased from Sigma-Aldrich (Milano, Italy). Deuterated phosphate buffer (pH 7.4; 1.5 M) with 10% TSP was prepared and used for pH adjustment.
4
NMR-based Metabolite Profiling of Cod Muscle
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A selection of water-soluble metabolites (TMA: trimethylamine, IMP: inosine monophosphate, HxR: Inosine, and Hx: hypoxanthine) were analysed by nuclear magnetic resonance (NMR) according to the method described by Shumilina, et al. [36 (link)]. The metabolites were extracted from 5 g cod muscle using 7.5% trichloroacetic acid (TCA, Sigma-Aldrich), as described previously [36 (link)]. 1D 1H-NMR-spectra were acquired at 300 K using a Bruker Avance 600-MHz spectrometer equipped with a 5 mm z-gradient TXI (H/C/N) cryoprobe (Bruker, Billerica, MA, USA). The NMR analysis and subsequent spectra processing were performed at the NMR centre of the faculty of Natural Sciences at NTNU (Trondheim). Spectra were processed using the TopSpin 3.5 software (Bruker)), and Trimethylsilyl propanoic acid (TSP, Sigma-Aldrich) was used as an external standard for spectral calibration (0 ppm). The reported concentrations are based on an average integral value derived from three integrations of the same resonance signal.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!