Ab82814

Manufactured by Abcam

Sourced in United States

Ab82814 is a primary antibody product offered by Abcam. It is a polyclonal antibody raised in rabbit and recognizes an unspecified target.

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Lab products found in correlation

3 protocols using ab82814

PrP Detection Utilizing Antibodies

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EFV was purchased from Toronto Research Chemicals Inc., Ontario, Canada. The PK and Pefabloc (protease inhibitor) were purchased from Roche, Germany. All other reagents/chemicals were purchased from Sigma-Aldrich, USA. The PrP monoclonal antibody (mAb) 4H11 used in this study to detect PrP has been previously described [42 (link)]. The antibodies anti-Cyp46A1 (ab82814, Abcam, USA), anti-flotillin-1 (610,821, Mouse BD Transduction Laboratories) and anti-β-actin (Cell signaling) were used. Secondary antibody conjugated with peroxidase, goat anti-mouse HRP, was obtained from Jackson Immuno Research, USA.

Ali T., Hannaoui S., Nemani S., Tahir W., Zemlyankina I., Cherry P., Shim S.Y., Sim V., Schaetzl H.M, & Gilch S. (2021). Oral administration of repurposed drug targeting Cyp46A1 increases survival times of prion infected mice. Acta Neuropathologica Communications, 9, 58.

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Cholesterol Metabolism Regulation

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27-Hydroxycholesterol was provided by American Santa Cruz, and 27-OHC (10 mg) was completely dissolved in a suitable amount of ethanol, then divided equally in centrifuge tubes, with nitrogen gas blowing dry, and finally preserved at −80°C. The 27-OHC working solution contains 0.16% ethanol (v/v). Filipin III was from Cayman Chemical (#70440, Ann Arbor, MI, United States). Primary antibodies for Western blot included mouse anti-ABCA1 (Abcam, ab66217), rabbit anti-ABCG1 (Abcam, ab52617), mouse anti-Caveolin-1 (Abcam, ab17052), rabbit anti-Apolipoprotein E (Abcam, ab52607), mouse anti-LDLR (Millipore, MABS26), rabbit anti-ACAT1 (Abcam, ab168342), rabbit anti-CYP46A1 (Sigma–Aldrich, SAB2100523), mouse anti-KDEL (Santa Cruz, sc-58774), rabbit anti-flotillin (Abcam, ab41927), rabbit anti-SR-B1 (Abcam, 52629), mouse anti- N Cadherin (Abcam, ab98952), mouse anti-Aβ (Abcam, ab126649), and rabbit anti-LRP1 (Abcam, ab92544). Goat anti-mouse (#14709) and rabbit (#7074) biotinylated secondary antibodies were from Cell Signaling Technology. Antibodies for immunofluorescence included anti-CYP46A1 (Abcam, ab82814), goat anti-mouse (Abcam, ab150120), and goat anti-rabbit (Abcam, ab96899).

Wang Y., Zhang X., Wang T., Liu W., Wang L., Hao L., Ju M, & Xiao R. (2020). 27-Hydroxycholesterol Promotes the Transfer of Astrocyte-Derived Cholesterol to Neurons in Co-cultured SH-SY5Y Cells and C6 Cells. Frontiers in Cell and Developmental Biology, 8, 580599.

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Subcellular fractionation and protein analysis

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Subcellular fractionation and Western blot analysis were performed at postnatal age p21 as described in Gardoni et al. (2006; 2001) . After the Triton-soluble fraction (TSF) and Tritoninsoluble fraction (TIF) were prepared, the latter was thoroughly tested for the absence of the presynaptic marker synaptophysin and for the presence of post-synaptic marker PSD95.
Membrane-associated Cyp46 (Pasciuto et al., 2015) and transferrin receptor, respectively, were used as normalisers. The following specific antibodies were used for Western blotting:
synaptophysin (abcam ab8049), cyp46 (ab82814), Fmrp (Ram II), transferrin R (Abcam ab84036), GABA A α2 (Abcam ab72445), GABA A ß1 (Abcam ab16703), GABA A δ (Millipore Ab9752), PSD95 (Thermo Scientific 6G6-1C9).

Sabanov V., Braat S., D'Andrea L., Willemsen R., Zeidler S., Rooms L., Bagni C., Kooy R.F, & Balschun D. (2017). Impaired GABAergic inhibition in the hippocampus of Fmr1 knockout mice. Neuropharmacology, 116.

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