Vero cells were infected with VACV recombinants expressing SARS-CoV-2 spike protein or RBD. Cell lysates were harvested 24 h post-infection; samples were boiled for 10 min, separated in a 10% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) gel, and transferred onto nitrocellulose filter membranes. After being blocked with 5% milk, membranes were probed with the antibodies anti-SARS-CoV-2 S2 (GTX632604, GeneTex, Irvine, CA, USA), anti-SARS-CoV-2 RBD (Sino Biological Inc., Beijing, China; 40592-T62), and anti-actin (Sigma-Aldrich, St. Louis, MO, USA; A1978) followed by incubation with IRDye 800-labeled IgG and IRDye 680-labeled IgG secondary antibodies (Li-Cor Biosciences, Lincoln, NE, USA). Protein signals were scanned with Odyssey (Li-Cor Biosciences, Lincoln, NE, USA).
Anti sars cov 2 s2
Manufactured by GeneTex
Sourced in United States, Taiwan, Province of China
The Anti-SARS-CoV-2 S2 is a lab equipment product that detects the S2 subunit of the SARS-CoV-2 spike protein. It is used for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Anti actin, by Merck Group (1 mentions)
Anti sars cov 2 rbd, by Sino Biological (1 mentions)
Irdye 800 labeled igg, by LI COR (1 mentions)
Irdye 680 labeled igg secondary antibodies, by LI COR (1 mentions)
Camostat mesylate, by Merck Group (1 mentions)
Amphotericin b solution, by Merck Group (1 mentions)
Anti aldolase a, by Santa Cruz Biotechnology (1 mentions)
Ivermectin, by Merck Group (1 mentions)
Bapta am, by Merck Group (1 mentions)
Trypsin, by Merck Group (1 mentions)
Anti beta actin, by GeneTex (1 mentions)
Pngase f, by New England Biolabs (1 mentions)
Supersignal west pico plus chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions)
Anti β actin, by Merck Group (1 mentions)
4 protocols using anti sars cov 2 s2
1
SARS-CoV-2 Spike Protein Detection
2
SARS-CoV-2 Spike Pseudovirus Infection Assay
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A6-001 (ChemDiv; Cat# F575-0482, N-(4-fluorobenzyl)-3-((6-phenylpyridazin-3-yl)amino)benzamide), A6-004 (ChemDiv; Cat# F474-2423, ethyl 8-methyl-4-((4-(methylthio)benzyl)amino)-2-oxo-1,2-dihydroquinoline-3-carboxylate), abamectin (Cayman Chemical, Ann Arbor, MI, USA), ivermectin (Sigma-Aldrich, St. Louis, MO, USA), camostat mesylate (Sigma-Aldrich, #SML0057), BAPTA-AM (Sigma-Aldrich, #A1076), amphotericin B solution (Sigma-Aldrich, #A2942), and trypsin (Sigma-Aldrich, #T6567) were purchased commercially. Lentifect™ SARS-CoV-2 Spike-pseudotyped Lentivirus used in the single-round infection (GFP reporter) was commercially acquired from Genecopoeia (cat# SP001). The following antibodies were acquired commercially: anti-SARS-CoV-2 S2 (GeneTex #GTX632604), anti-aldolase A (Santa Cruz Biotechnology #SC-390733), and anti-HIV-p24 (GeneTex #GTX64128).
3
SARS-CoV-2 Spike Protein Detection
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Cells were lysed in lysis buffer (50 mM Tris, 250 mM NaCl, 3 mM EDTA, 10% Triton X-100, 0.5% NP-40, and 10% glycerol). Cell lysate (10–50 μg) or viral supernatant (equal volume) were subjected to immunoblotting. Primary antibodies used in the study were anti-SARS-CoV-2 S2 (Genetex, Hsinchu, Taiwan; GTX632604), anti-VSV-M (Absolute, Boston, MA), and anti-beta-actin (Genetex, Hsinchu, Taiwan). The RBD antiserum was collected from rabbits immunized with RBD recombinant protein purified from E. coli. To remove N-linked oligosaccharides, viral supernatants were treated with peptide N-glycosidase F (PNGase F; NEB, Ipswich, MA) at 37°C for 1 h and then subjected to immunoblotting.
4
SARS-CoV-2 Spike Protein Detection Assay
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Cells were lysed by lysis buffer (50 mM Tris, 250 mM NaCl, 3 mM EDTA, 1% Triton X-100, 0.5% NP-40, 10% Glycerol, 1x PI cocktail). Cell lysate (10-50 μg) or viral supernatant were subjected to SDS-PAGE and transferred onto PVDF membrane. The primary antibodies were used in the study: anti-SARS-CoV-2 S2 (Genetex, Hsinchu, Taiwan; Cat#632604), anti-VSV-M (Absolute, Boston, MA), and anti-beta-actin (Sigma, St. Louis, MO). The rabbit anti-RBD (RBD antigen purified from E.coli) was generated in-house. The secondary antibodies were purchased from Jackson immunoresearch (West Grove, PA). The detection signals were developed with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Scientific, Rockford, IL) and imaged by Amersham Imager 600 (Dealer GE Healthcare).
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