No colorimetric assay kit

Manufactured by Beyotime

Sourced in China

The NO colorimetric assay kit is a laboratory instrument designed to quantify nitric oxide (NO) levels in biological samples. It provides a reliable and accurate method for measuring NO concentration.

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Lab products found in correlation

Microplate reader, by Bio-Rad (1 mentions)

Spelling variants of this product

Colorimetric assay kit (35 citations) Colorimetric assay (8 citations) NO kits (3 citations)

2 protocols using no colorimetric assay kit

Quantifying Lung Nitric Oxide Levels

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The lower lobes of the right lung from each group were assessed for NO levels, analyze
the concentrations of NO in lung tissue with a NO colorimetric assay kit according to the
Griess reaction (Beyotime Institute of Biotechnology, China).The concentration of NO was
calculated based on the absorbance calculated at 540 nm and standardized by the total
protein’s amount of each sample.

Dong Y., Zhang L., Jiang Y., Dai J., Tang L, & Liu G. (2019). Emodin reactivated autophagy and alleviated inflammatory lung injury in mice with lethal endotoxemia. Experimental Animals, 68(4), 559-568.

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Quantifying Inflammatory Mediators in Macrophages

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After treatment of LPS for 24 h, the supernatant of macrophages medium was collected. NO release was determined by NO Colorimetric Assay Kit (Beyotime, Shanghai, China) and the absorbance at 540 nm was measured with a microplate reader (Bio-Rad, Hercules, CA, USA). Levels of TNF-α, IL-1β and IL-6 in medium were detected by special commercial enzyme-linked immunosorbent assay (ELISA) kits (Sigma, St. Louis, MO, USA) according to the manufacturer’s instructions. The abundance of inflammatory cytokine was calculated by the absorbance at 450 nm and a standard curve.

Li Y., Guo W, & Cai Y. (2020). NEAT1 Promotes LPS-induced Inflammatory Injury in Macrophages by Regulating MiR-17-5p/TLR4. Open Medicine, 15, 38-49.

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