Ethanol (>99.8%) was purchased from Merck and type II water was used as an extraction solvent for the solid–liquid extraction process. Thrombin 6 receptor activating peptide (TRAP-6), Adenosine 5′-diphosphate (ADP), and collagen were obtained from Sigma-Aldrich (St. Louis, MO, USA). Sodium citrate vacuum tubes were purchased from Genetics and Technology spa (GENYTEC), Santiago of Chile, Chile. Anti-CD62P-PE, anti-CD61-FITC, and anti-CD63-PE antibodies were purchased from BD Pharmingen (BD Biosciences, San Diego, CA, USA). The HPLC grade solvents used were purchased from Burdick and Jackson (Muskegon, MI, USA).
Adenosine 5 diphosphate
Manufactured by Merck Group
Sourced in United States
Adenosine 5′-diphosphate is a chemical compound that is a nucleotide composed of adenosine and two phosphate groups. It is a key intermediate in cellular energy transfer processes.
Automatically generated - may contain errors
Lab products found in correlation
Adenosine 5 triphosphate, by Merck Group (5 mentions)
Adenosine 5 monophosphate, by Merck Group (5 mentions)
Paraformaldehyde (pfa), by Merck Group (3 mentions)
Pyruvate, by Merck Group (3 mentions)
Bovine serum albumin (bsa), by Merck Group (3 mentions)
Oligomycin a, by Merck Group (2 mentions)
Milli q system, by Merck Group (2 mentions)
Triton x 100, by Merck Group (2 mentions)
Tepp 46, by Aobious (2 mentions)
Phosphoenolpyruvic acid monopotassium salt b20358, by Thermo Fisher Scientific (2 mentions)
13c6 glucose clm 1396 13c5 glutamine clm 1822 3 3 3 d3 lactate dlm 9071 3 13c pyruvate clm 1585, by Cambridge Isotopes (2 mentions)
13c3 pyruvate clm 2440, by Cambridge Isotopes (2 mentions)
Mtbstfa 1 tbdmcs ts 48927, by Thermo Fisher Scientific (2 mentions)
Purified recombinant hldha 6374, by Abcam (2 mentions)
Purified recombinant hldhb 6375, by Abcam (2 mentions)
Uridine 5 triphosphate, by Merck Group (2 mentions)
Uridine 5 diphosphate, by Merck Group (2 mentions)
Rotenone, by Merck Group (2 mentions)
Malate, by Merck Group (2 mentions)
β nicotinamide adenine dinucleotide, by Merck Group (2 mentions)
19 protocols using adenosine 5 diphosphate
1
Platelet Activation Assay Protocol
2
Biochemical Reagents for Protein Analysis
3
Tetramethylpyrazine Inhibits Platelet Activation
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Tetramethylpyrazine (TMP) was obtained from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Ticagrelor (Tica) was from Selleck (Shanghai, China). Adenosine 5'-diphosphate (ADP) and MRS2179 (P2Y1 receptor antagonist) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Thrombin was from Beijing Solarbio Science & Technology Co., Ltd. (Bejing, China). Dimethyl sulfoxide (DMSO) was from Amresco (Houston, Texas, USA). Antibodies (anti-CD61-APC, anti-CD62P-phycoerythrin-labeled (PE), and anti-GPIIb/IIIa-FITC PAC-1) were purchased from BD Biosciences (San Diego, CA, USA). SQ22536 (an AC inhibitor) and LY294002 (a PI3k inhibitor) were obtained from APExBIO Technology LLC (Houston, Texas, USA). The cyclic AMP enzyme immunoassay (ELISA) kit was from Cayman Chemical (Ann Arbor, MI, USA). The IL-1β and soluble CD40 ligand (sCD40L) ELISA kits were obtained from Elabscience Biotechnology Co., Ltd. (Wuhan, China). The bicinchoninic acid (BCA) protein assay kit was from Beyotime (Shanghai, China). Antibodies against phospho-VASPser157, Akt, phospho-Aktser473, and phospho-AktThr308 were acquired from Cell Signaling Technology (Beverly, MA, USA). Tetramethylpyrazine, SQ22536, and LY294002 were dissolved in DMSO for further use in experiments. DMSO 0.1% was employed as the vehicle.
4
Nucleotide Compounds for Biochemical Assays
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Adenosine 5′-triphosphate (ATP), γ-β-methyleneAdenosine 5′-triphosphate (ATPγC), and Adenosine 5′-diphosphate (ADP) were purchased from Sigma Aldrich (St. Louis, MO, USA). Adenosine 5′-(β,γ-imido)triphosphate (ATPγN) was purchased from Roche Diagnostics (Indianapolis, IN, USA)
5
Platelet Function Assays Protocol
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Adenosine 5′-diphosphate (ADP), Hoechst 33258, Poly-L-lysine solution, paraformaldehyde (PFA), eosin-hematoxylin solution, Triton X-100, tetramethyrhodamine isothiocyanate (TRITC)-conjugated phalloidin and cyclopiazonic acid from Penicillium cyclopium (CPA) were from Sigma Aldrich (Milan, Italy). Fura-2 acetoxymethyl ester (Fura-2 AM) was from Molecular Probes Europe BV (Leiden, The Netherlands). Human fibronectin was from BD Bioscience (Milan, Italy). Type I collagen was purified as described previously35 (link). The following antibodies were used: monoclonal anti-CD61 (clone SZ21) (Immunotech, Marseille, France); goat monoclonal anti-CD61 (clone C-20) and mouse monoclonal anti-P-Selectin (clone 1E3) (Santa Cruz Biotechnology, California, USA); mouse monoclonal anti-α-tubulin (clone DM1A) and mouse monoclonal anti-human P-Selectin-allophycocyanin (APC) (clone clone AK4) (Sigma Aldrich, Milan, Italy); rabbit polyclonal anti-von Willebrand Factor (Dako, Milan, Italy); mouse monoclonal anti-Thrombospondin (clone A6.1), mouse monoclonal anti-CD61-fluorescein isothiocyanate (FITC) (clone PM6/13) and mouse monoclonal anti-CD42b-phycoerythrin (PE) (clone HIP1) (Abcam, Cambridge, UK); anti-CD61-FITC and anti-CD42b-PE (BD Biosciences, San José, CA, USA); Alexa Fluor-conjugated antibodies (Invitrogen, Milan, Italy).
6
Metabolic Flux Analysis Protocol
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DASA was purchased from EMD Millipore (550602) and TEPP-46 was purchased from Aobious (AOB1123). Pyridoxamine dihydrochloride (P9380), beta-nicotinamide adenine dinucleotide, reduced (N8129), 13C6 citrate (606081), oxaloacetic acid (O4126), l-aspartic acid (A9256), methoxamine (M6524), pyridine (270970) and adenosine 5-diphosphate (A2754) were purchased from Sigma. Phosphoenolpyruvic acid monopotassium salt (B20358) was purchased from Alfa Aesar. 13C6 glucose (CLM-1396), 13C5 glutamine (CLM-1822), 3,3,3-D3 lactate (DLM-9071), 3-13C pyruvate (CLM-1585), and 13C3 pyruvate (CLM-2440) were purchased from Cambridge Isotope Laboratories. D-glucose (50146244), dimethylformamide (P120673), MSTFA + 1% TMCS (TS-48915) and MTBSTFA + 1% TBDMCS (TS-48927) were purchased from Fisher. Purified recombinant hLDHA (6374) and purified recombinant hLDHB (6375) were purchased from Biovision. Purified rbLDHA was purchased from VWR EMD (80601).
7
Adenosine Metabolism Quantification
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Adenosine was purchased from Calbiochem (Merck Millipore). Adenosine 5′-diphosphate (ADP), adenosine 5′-monophosphate (AMP), adenosine 3′,5′-cyclic monophosphate (AMPc), inosine, Erythro-9(-2-hydroxy-3-nonyl)adenine (EHNA), dipyridamol and dextrose were purchased from Sigma-Aldrich (France). FAAH enzymes were from Cayman Chemical. Ethanol was from Carlo-Erba (France). [2,8-3H]-Adenosine and [2,8-3H]-Adenosine 5′-triphosphate tetraammonium salt were obtained from Moravek Biochemicals (Brea, CA). Fluorescent probes CholEsteryl BODIPY® FL C12 and CholEsteryl BODIPY® 542/563 C11 were obtained from Life Technologies. Ultra-pure water was prepared using a MilliQ system (Millipore Corporation, Billerica, MA). Soluene, Ultima Gold and Hionic-Fluor were purchased from Perkin Elmer (France). Reagents and HPLC grade solvents as methanol, ethyl acetate, acetonitrile, and ammonium hydroxyde (NH4OH) were provided by Carlo Erba (France). Potassium hydrogen phosphate (KH2PO4) and tetrabutylammonium chloride (TBAC) were from Fluka, ammonium acetate (CH3COONH4) from VWR and formic acid from Sigma-Aldrich (France). Liquid scintillator cocktails were provided from Perkin Elmer (France). Cartridges for SPE were from Phenomenex (France), and 0.45 μm Millex filters were from Millipore (France).
8
Elucidating Purinergic Signaling Mechanisms
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Suramin and Reactive-Blue-2 (RB-2) were obtained from MP Biomedical (Santa Ana, CA, United States). DMEM/F12, advanced DMEM/F12, HEPES, L -glutamine, penicillin, streptomycin, FBS, Dulbecco’s PBS, apyrase, formyl-methionine-leucyl-phenylalanine (FMLP), LPSs, adenosine 5’-triphosphate (ATP), adenosine 5’-diphosphate (ADP), uridine 5’-triphosphate (UTP), uridine 5’-diphosphate (UDP), adenosine, Zm 241385, and fibroblast growth factor 2 (FGF2) were purchased from Sigma–Aldrich (Oakville, ON, Canada). Collagenase type I, SuperScript III, gentamicin, B-27 and N-2 supplements, polyinosinic–polycytidylic acid [poly(I:C)], EDTA, and TRIzol were obtained from Invitrogen (Carlsbad, CA, United States). Collagen type I was purchased from BD Bioscience (San Jose, CA, United States). Y-27632, mrEGF, Wnt-3a, and R-spondin were purchased from R&D Systems (Minneapolis, MN, United States). Noggin and M-CSF were purchased from PeproTech (QC, Canada). SYBR Green and DNAseI were from Roche Diagnostics (Indianapolis, IN, United States). Flagellin was obtained from InvivoGen (San Diego, CA, United States). Oligo(dt)18 was obtained from Fisher Scientific (Ottawa, ON, Canada). MRS 2500, MRS 2179, MRS 2578, AR-C 118925XX, PSB 1114, and 5-BDBD were purchased from Tocris Bioscience (Minneapolis, MN, United States).
9
Mitochondrial Bioenergetics Profiling Protocol
10
Platelet Functional Assays and Flow Cytometry
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For platelet counts, peripheral blood was collected from the retro-orbital sinus using EDTA coated tubes, and assessed using a Hemavet Analyzer (Drew Scientific, FL). For functional platelet assays, blood was collected into heparinized tubes (BD Biosciences, San Jose, CA). Platelet-rich plasma was prepared as previously described (REF). For flow cytometry, 50 uL of whole blood was added to 200 uL Tris-buffered saline (20mM Tris-HCl, 137mM NaCl). After further dilution with 1 mL of 2 mM CaCl2 in modified Tyrodes-HEPES buffer (5mM HEPES, 140mM NaCl, 2.7mM KCl, 5.5mM dextrose, 0.42mM Na2HPO4, 12mM NaHCO3), platelets were stained with anti-GPIIb/IIIa (CD41/61) FITC together with anti-GPIIb/IIIa (CD41/61) PE (JONA clone) or anti-P-selectin PE (Emfret, Würzburg, Germany). During staining, platelets were also stimulated with 1mM adenosine 5-diphosphate (ADP; Sigma-Aldrich, St. Louis, MO, USA), 0.1U or 0.01U Thrombin (Roche, Basel, Switzerland), or 3mM Thromboxane A2 analog U46619 (Cayman Chemical, Ann Arbor, MI, USA) at 37°C for 20min. Reactions were stopped by adding 300 ul 0.5% PFA (in PBS) to the samples and analyzed using a FACS Calibur cytometer (BD Biosciences, San Jose, CA, USA) and FlowJo software (TreeStar, Ashland, OR, USA).
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