Waters 2489 uv detector

Manufactured by Waters Corporation

Sourced in United States, China

The Waters 2489 UV detector is a laboratory instrument used for the detection and analysis of compounds in liquid chromatography (LC) systems. It measures the absorbance of light by the sample at a specific wavelength, which can be used to identify and quantify the presence of different molecules in a mixture.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Waters 2489 (38 citations) UV detector (22 citations) Waters 2489 UV/Visible detector (19 citations) 2489 UV detector (18 citations) Waters 2489 UV-Vis detector (14 citations) Waters 2489 detector (3 citations) Waters 2489 UV (2 citations)

18 protocols using waters 2489 uv detector

Extraction and Characterization of Concentrated Grape Juice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Concentrated grape juice: Natural grape juice obtained from squeezing of Syrah or Shiraz, Cabernet Sauvignon and Malbec grapes was placed in the evaporation equipment, which is a flat, spiral, or tubular heat exchanger (concentrated grape juice is purchased from Shangri‐La Wine Co., Ltd.). The grape juice was heated in the evaporator and concentrated to 40 Brix. The concentrated grape juice was placed in containers and stored in a freezer at 15°C before use (Juchuang Environmental Protection Equipment Co., Ltd., Haier Group Co., Ltd.). After LC‐UV method and calibration of the standard curve were performed, the tartaric acid content in the concentrated grape juice was found to be 17.68%.
Reagents: NaOH, HCl, NaCl, KH₂PO₄, and acetonitrile were purchased from Xilong Chemical Co., Ltd. All of the chemicals used were of chromatographic reagent grade. Double‐distilled water was used throughout the study.
The following instruments were used: Waters 1525 Chromatograph and Waters 2489 UV‐detector (Waters Co., Ltd.), 335 anion‐exchange resins, D‐314 anion‐exchange resin, and D‐914 anion‐exchange resin with 150 mm × 4.6 mm and 5 µm particle size (Waters Atlantis T3).

Li N., Wei Y., Li X., Wang J., Zhou J, & Wang J. (2019). Optimization of deacidification for concentrated grape juice. Food Science & Nutrition, 7(6), 2050-2058.

+ Open protocol

+ Expand

Size Exclusion Chromatography of Biomolecules

Check if the same lab product or an alternative is used in the 5 most similar protocols

LC separation was performed on a Waters2695 system with a TSK-GEL G3000 SWXL column (300 mm × 7.8 mm, Tosoh, Japan). The injection volume was 50 μL. The flow rate was 0.5 mL/min using an elution buffer of 40 mM phosphate buffer containing 300 mM sodium sulfate (pH 7.2) and the column temperature was maintained at 25 °C. The detection was performed on a Waters2489 UV detector (Waters, Milford, MA, USA) at 280 nm. Data were acquired and processed using Waters Empower (Waters Corporation, Milford, MA, USA). The relative percentage contents were calculated by the area normalization method.

Yu L., Li Y., Tao L., Jia C., Yao W., Rao C, & Wang J. (2019). Identification of a Recombinant Human Interleukin-12 (rhIL-12) Fragment in Non-Reduced SDS-PAGE. Molecules, 24(7), 1210.

+ Open protocol

+ Expand

HPLC Analysis of Fermentation Products

Check if the same lab product or an alternative is used in the 5 most similar protocols

High-Performance Liquid Chromatography (HPLC) method was used to measure the contents of ethanol and by-products. The parameters for the measurement of glucose, ethanol, and glycerol contents were a mobile phase of 0.01 mol/L H₂SO₄, a flow rate of 0.8 mL/min, column temperature of 50 °C with the instruments of Waters 1525 binary HPLC pump, Waters 2410 refractive index detector, and Shodex SH1011 chromatographic column [29 (link)]. In addition, the parameters for the content determination of organic acids, mainly including lactic acid, acetic acid, and succinic acid were detection wavelength of 210 nm, a mobile phase A of 10 mM KH₂PO₄, a mobile phase B of methanol, flow rate of 1.0 mL/min, column temperature of 30 °C with the instruments of Waters Alliance E2695, Waters 2489 UV detector, and Waters XSelect HSS chromatographic column [30 (link)]. The concentration of biomass in the medium was measured by the gravimetric method [31 (link)].

Yang P., Jiang S., Lu S., Jiang S., Jiang S., Deng Y., Lu J., Wang H, & Zhou Y. (2022). Ethanol yield improvement in Saccharomyces cerevisiae GPD2 Delta FPS1 Delta ADH2 Delta DLD3 Delta mutant and molecular mechanism exploration based on the metabolic flux and transcriptomics approaches. Microbial Cell Factories, 21, 160.

+ Open protocol

+ Expand

HPLC Analysis of Organic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

A Waters High performance liquid chromatography (HPLC) system equipped with a Water 600 binary pump, Waters 2489 UV/detector, and Waters 717 plus autosampler (Waters Technologies Corporation, 34 Maple St., Milford, MA 0157) and a Phenomenex Luna 5um C₁₈ (2) 250 × 4.6 mm column (Torrance, CA, USA) were used at a detection wavelength of 273 nm. The mobile phase consisted of Methanol and water at a ratio of 70:30 (v/v). The mobile phase flow rate was maintained at 1.0mL/min. and an injection volume of was 20 μL was used (O’connell and Zurzola, 1984 (link)). HPLC data was analyzed using Empower V. software (Milford, MA, USA).

Pimparade M.B., Morott J.T., Park J.B., Kulkarni V.I., Majumdar S., Murthy S.N., Lian Z., Pinto E., Bi V., Durig T., Murthy R., Shivakumar H.N., Vanaja K., Kumar C.P, & Repka M.A. (2015). Development of taste masked caffeine citrate formulations utilizing hot melt extrusion technology and in vitro-in vivo evaluations. International journal of pharmaceutics, 487(0), 167-176.

+ Open protocol

+ Expand

HPLC Quantification of Salidroside in ATWE

Check if the same lab product or an alternative is used in the 5 most similar protocols

The amount of salidroside in ATWE was quantified using HPLC. Briefly, 100 mg of ATWE was dissolved in 25 ml DW under sonication and methanol was added to make it 50 ml. The resulting solution was filtered through a 0.45-µm filter, and 10 µl was separated in a Cadenza CD-C18 (4.6 mm × 300 mm x 5 µm) column (Imtakt, United States) at 25°C in a Waters HPLC system (a 1525 binary pump with a 1500CH column oven and a 2027 auto sampler, Waters, United States) with detection at 220 nm using a Waters 2489 UV detector and the total runtime of 40 min. Gradient elution was performed with DW (A) and a 1:1 mixture of methanol/isopropanol (B) in the following manner (A:B in percentage): 95%: 5% for 0–40 min except for 20%: 80% for 20–25 min. As the standard, 80–200 µg/ml of salidroside in methanol was used.

Park J.S., Kwon E., Kim Y.S., Kim S.M., Kim D.S., Jang J.J., Yun J.W, & Kang B.C. (2021). Safety Assessment of Acer tegmentosum Maxim. Water Extract: General Toxicity Studies in Sprague–Dawley Rats and Beagle Dogs With Re-evaluation of Genotoxic Potentials. Frontiers in Pharmacology, 12, 687261.

+ Open protocol

+ Expand

Structural Characterization of Compound 1

Check if the same lab product or an alternative is used in the 5 most similar protocols

Optical rotations were determined with an MCP 200 modular circular polarimeter (Anton Paar Opto Tec GmbH, Seelze, Germany). UV spectra were recorded on a GENESYS 10S UV-Vis spectrophotometer (Thermo Fisher Scientific, Shanghai, China), and HR-ESIMS was obtained on an LC-ESIMS system in the positive-ion mode (Bruker Daltonics, Bremen, Germany). NMR spectra were measured on a Bruker AV-400 NMR spectrometer. Preparative HPLC was carried out on C18 reversed-phase columns (YMC, 250 × 10 mm i.d., 5 μm) using a Waters 2535 pump equipped with a Waters 2489 UV detector (Waters Corporation, Milford, MA, USA). Single-crystal X-ray diffraction analysis of 1 was performed on an Agilent Xcalibur Atlas Gemini Ultra-diffractometer with mirror monochromated CuKα radiation (λ = 1.54184 Å) at 150 K. Silica gel (100–200 mesh) (Qingdao Mar. Chem. Ind. Co. Ltd., Qingdao, China) and C18 reversed-phase silica gel (50 μm, YMC Co. Ltd., Kyoto, Japan) were used for column chromatography.

Zhang Q., Satyanandamurty T., Shen L, & Wu J. (2017). Krishnolides A–D: New 2-Ketokhayanolides from the Krishna Mangrove, Xylocarpus moluccensis. Marine Drugs, 15(11), 333.

+ Open protocol

+ Expand

Spectroscopic Characterization of Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Optical rotations were measured on a MCP200 modular circular polarimeter (Anton Paar OptoTec GmbH, Seelze, Germany). UV spectra were obtained on a GENESYS 10S UV–Vis spectrophotometer (Thermo Fisher Scientific, Shanghai, China). NMR spectra were recorded on a Bruker AV-400 spectrometer (Bruker Scientific Technology Co. Ltd., Karlsruhe, Germany) with TMS as the internal standard. HR-ESIMS were measured on a Bruker maXis ESI-QTOF mass spectrometer (Bruker Daltonics, Bremen, Germany). Semi-preparative HPLC was performed on C₁₈ reversed-phase silica gel columns (YMC 250 × 10 mm i.d., or 250 × 4.6 mm i.d.) using a Waters 2535 pump equipped with a Waters 2489 UV detector (Waters Corporation, Milford, MA, USA). Silica gel (100–200 mesh) (Qingdao Marine Chemical Industrial Co. Ltd., Qingdao, China) and C₁₈ reversed-phase silica gel (ODS-A-HG 12 nm, 50 μm, YMC Co. Ltd., Kyoto, Japan) were used for column chromatography. ECD spectra were measured on a Jasco J-810 spectropolarimeter (JASCO Corporation, Tokyo, Japan) in MeCN.

Ren J.L., Zou X.P., Li W.S., Shen L, & Wu J. (2018). Limonoids Containing a C1–O–C29 Moiety: Isolation, Structural Modification, and Antiviral Activity. Marine Drugs, 16(11), 434.

+ Open protocol

+ Expand

HPLC Analysis of Ketoprofen Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

All samples were analyzed using a Waters HPLC equiped with Empower software to analyze the data. HPLC consisted of a Water 600 binary pump, Waters 2489 UV/detector, and Waters 717 plus autosampler (Waters Technologies Corporation, 34 Maple St., Milford, MA 0157). The column used was phenomenex luna C18 (5µ, 250 mm × 4.6 mm). The mobile phase constituted of acetonitrile/20 mMol phosphate buffer, 55:45 (%v/v) at pH 4^{30 (link), 31 (link)} at a flow rate of 1 ml/min and injection volume of 20 µl. The UV detetctor wavelength for KTP detection was set at 256 nm.

Ashour E.A., Kulkarni V., Almutairy B., Park J.B., Shah S., Majumdar S., Lian Z., Pinto E., Bi Y., Durig T., Martin S.T, & Repka M.A. (2015). Influence of Pressurized Carbon Dioxide on Ketoprofen-Incorporated Hot-Melt Extruded Low Molecular Weight Hydroxypropylcellulose. Drug development and industrial pharmacy, 42(1), 123-130.

+ Open protocol

+ Expand

HPLC Quantification of Sildenafil and Glycyrrhizin

Check if the same lab product or an alternative is used in the 5 most similar protocols

Waters e2695 HPLC system that contains a Waters 600 binary pump, Waters® 717 plus autosampler, and Waters 2489 UV/detector (Waters Technologies Corporation, Milford, MA, USA). In addition, the Xselect HSS cyano column (250 mm × 4.6 mm, 5 μm) was used for the quantification of both sildenafil and glycyrrhizin. A mobile phase consisting of triethanolamine (1 %, pH was adjusted to 3.9 by formic acid), acetonitrile, and water at a gradient elution starting from 60 %, 40 % and 0 %, respectively, from 0 to 2 min, followed by 0 %, 40 %, 60 %, respectively, from 2 to 5 min and finally, 60 %, 40 % and 0 %, respectively, from 0 to 2 min was used for the separation of the loaded materials. The flow rate was set as 1.5 mL/min, the temperature was adjusted at 21 °C, the volume of injection was 10 μL, and the detection wavelength was 254 nm. Both sildenafil and glycyrrhizin were dissolved in 85 % (v/v) PBS and 15 % (v/v) ethanol to prepare the stock solution. The calibration curve was determined by using the above method in which the serial dilution was used in the range between 144 and 0.5 μg/mL. The retention times (Rt) of glycyrrhizin and sildenafil were determined at 2.3 and 6.2 min, respectively, as shown in the Supplementary Materials Section – Figure S2. The data analysis and the calibration curve were plotted by OriginPro® 2021 software (OriginLab Corporation, Northampton, MA, USA).

Alamer A.A., Alshehri A.A., Aodah A.H., Almughem F.A., Alghmadi H.A., Alali A.S., Halwani A.A., Muqtader Ahmed M, & Tawfik E.A. (2024). Development and evaluation of sildenafil/glycyrrhizin-loaded nanofibers as a potential novel buccal delivery system for erectile dysfunction. Saudi Pharmaceutical Journal : SPJ, 32(5), 102038.

+ Open protocol

+ Expand

Organic Acid Quantification in Culture Broth

Check if the same lab product or an alternative is used in the 5 most similar protocols

To detect organic acid titer in culture broth, 1 mL of 2 M sulfuric acid was added into 1-mL well-mixed sample in a 15-mL tube and the mixture was incubated at 80 °C for 30 min. The mixture was vortexed at intervals to resolve the malate adequately. Subsequently, 2 mL of distilled water was added, mixed, and an aliquot was used for metabolite analysis.
Malic acid titer was determined by high-performance liquid chromatography (HPLC) using an instrument (e2695; Waters, Manchester, United Kingdom) equipped with an Aminex HPX-87H column (Bio-Rad, Hercules, CA, USA) at 35 °C and a Waters 2489 UV detector at 40 °C; 5 mM H₂SO₄ was used as the mobile phase with a constant flow rate of 0.5 mL/min. Sugar concentrations were monitored with a Waters 2414 refractive index detector and an Aminex HPX-87P column (Bio-Rad) with distilled water as the mobile phase. Data analysis was performed using a Waters e2695 separation module.

Li J., Chen B., Gu S., Zhao Z., Liu Q., Sun T., Zhang Y., Wu T., Liu D., Sun W, & Tian C. (2021). Coordination of consolidated bioprocessing technology and carbon dioxide fixation to produce malic acid directly from plant biomass in Myceliophthora thermophila. Biotechnology for Biofuels, 14, 186.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!