Dulbecco s modified eagle s medium dmem with glucose

Manufactured by Thermo Fisher Scientific

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Dulbecco's modified Eagle's medium (DMEM) with glucose is a cell culture medium formulation designed to support the growth and maintenance of a variety of cell types. It provides essential nutrients, amino acids, vitamins, and glucose to sustain cellular metabolism and proliferation.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions) 3h dopamine, by PerkinElmer (3 mentions) 3h serotonin, by PerkinElmer (3 mentions) Transit lt1, by Mirus Bio (3 mentions) Dulbecco s phosphate buffered saline dpbs, by Thermo Fisher Scientific (2 mentions) Scintillation fluid, by Thermo Fisher Scientific (2 mentions) Lipojet reagent, by Mirus Bio (2 mentions) Mtsea biotin, by Biotium (2 mentions) Non radiolabeled substrates, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (1 mentions) Desipramine, by Merck Group (1 mentions) Fluoxetine, by Merck Group (1 mentions) 3h norepinephrine, by PerkinElmer (1 mentions) 3h glutamic acid, by PerkinElmer (1 mentions) Paroxetine, by Merck Group (1 mentions) 3h gaba, by PerkinElmer (1 mentions) 3h glycine, by PerkinElmer (1 mentions) Phenol red, by Thermo Fisher Scientific (1 mentions) 2 7 dichlorofluorescin diacetate dcfh da, by Merck Group (1 mentions)

Spelling variants of this product

DMEM medium (4294 citations) Dulbecco’s modified (295 citations) DMEM-glucose (1 citations)

5 protocols using dulbecco s modified eagle s medium dmem with glucose

Radiolabeled Neurotransmitter Uptake Assays

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All experiments using animal subjects were conducted according to the National Institutes of Health Guide for the Care and Use of Laboratory Animals. All experiments involving animal subjects were conducted as preapproved by Drexel University Institutional Animal Care and Use Committees. Radio-labeled substrates, [³H]-dopamine (32.6 Ci/mmol) and [³H]-serotonin (23.9 Ci/mmol), were purchased from PerkinElmer (Boston, MA). Cell culture media and supplements, including penicillin/streptomycin, Dulbecco’s phosphate-buffered saline (D-PBS), Dulbecco’s modified Eagle’s medium (DMEM) with glucose, and scintillation fluid, were obtained from Thermo Fisher Scientific (Waltham, MA). Transfection reagents TransIT-LT1 and LipoJet reagent were from Mirus Bio LLC (Madison, WI) and SignaGen Laboratories (Rockville, MD), respectively. Reagents for uptake assays and nonradiolabeled substrates were purchased from Sigma-Aldrich (St. Louis, MO). MTSEA-biotin was purchased from Biotium, Inc. (Fremont, CA). Reagents for uptake assays and nonradiolabeled substrates were purchased from Sigma-Aldrich (St. Louis, MO).

Aggarwal S., Liu X., Rice C., Menell P., Clark P.J., Paparoidamis N., Xiao Y.C., Salvino J.M., Fontana A.C., España R.A., Kortagere S, & Mortensen O.V. (2019). Identification of a Novel Allosteric Modulator of the Human Dopamine Transporter. ACS chemical neuroscience, 10(8), 3718-3730.

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Radioactive Uptake Assay Protocol

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Sydnocarb was a gift from Teva Pharmaceuticals (Frazer, PA, USA). Radiolabeled substrates, [³H]-dopamine (32.6 Ci/mmol) and [³H]-serotonin (23.9 Ci/mmol), were purchased from PerkinElmer (Boston, MA, USA). Cell culture media and supplements, including penicillin/streptomycin, Dulbecco’s phosphate-buffered saline (DPBS), Dulbecco’s modified Eagle’s medium (DMEM) with glucose, and scintillation fluid, were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Transfection reagents TransIT-LT1 and LipoJet reagent were from Mirus Bio LLC (Madison, WI, USA) and SignaGen Laboratories (Rockville, MD, USA), respectively. Reagents for uptake assays and non-radiolabeled substrates were purchased from Sigma-Aldrich (St. Louis, MO, USA). MTSEA-biotin was purchased from Biotium, Inc. (Fremont, CA, USA). Reagents for uptake assays and non-radiolabeled substrates were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Aggarwal S., Cheng M.H., Salvino J.M., Bahar I, & Mortensen O.V. (2021). Functional Characterization of the Dopaminergic Psychostimulant Sydnocarb as an Allosteric Modulator of the Human Dopamine Transporter. Biomedicines, 9(6), 634.

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Radiolabeled Neurotransmitter Uptake Assay

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Non-radiolabeled serotonin and compounds fluoxetine, citalopram, desipramine, paroxetine, cocaine and amphetamine were purchased from Millipore-Sigma (Billerica, MA, USA).
D-PBS was purchased from Invitrogen Life Technologies (Carlsbad, CA, USA). Culture media for cell lines, including Dulbecco’s modified Eagle’s medium (DMEM) with glucose, fetal bovine serum and penicillin/streptomycin were obtained from Thermo Fisher Scientific (Waltham, MA, USA).
Transfection reagent TransIT-LT1 was from Mirus Bio LLC (Madison, WI, USA).
Radiolabeled substrates [³H]-dopamine (53.6 Ci/mmol), [³H]-serotonin (28.2 Ci/mmol), [³H]-norepinephrine (14.9 Ci/mmol), [³H]-glutamic acid (51.1 Ci/mmol), [³H]-glycine (48.7 Ci/mmol) and [³H]-GABA (92.1 Ci/mmol) were purchased from Perkin Elmer (Boston, MA, USA).
Scintillation fluid (Ecolite) was obtained from Thermo Fisher Scientific (Waltham, MA, USA).

Camicia F., Vaca H.R., Guarnaschelli I., Koziol U., Mortensen O.V, & Fontana A.C. (2022). Molecular characterization of the serotonergic transporter from the cestode Echinococcus granulosus: pharmacology and potential role in the nervous system. Parasitology research, 121(5), 1329-1343.

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Antioxidant Compounds and Cell Viability Study

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Taurine, methionine, vitamin E, vitamin D, malic acid, citric acid, rutin, quercetin, ferulic acid, and chlorogenic acid (98% pure) were obtained from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China), and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide (MTT), HEPES, 2′, 7′-dichlorofluorescin diacetate (DCFH- DA), and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich, Inc. (St. Louis, USA). Dulbecco’s modified Eagle’s medium (DMEM) with glucose, phenol red, fetal bovine serum (FBS), phosphate-buffered saline (PBS), penicillin, and streptomycin were purchased from Gibco Life Technologies (Grand Island, NY, USA). A bicinchoninic acid assay kit, trypsin, and NP-40 buffer were obtained from Beyotime Biotechnology Inc. (Beijing, China). All other chemicals used were of analytical grade and commercially available in China.

Wang Y., Zhang N., Zhou J., Sun P., Zhao L, & Zhou F. (2022). Protective Effects of Several Common Amino Acids, Vitamins, Organic Acids, Flavonoids and Phenolic Acids against Hepatocyte Damage Caused by Alcohol. Foods, 11(19), 3014.

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Metformin Modulates Cell Signaling

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Dulbecco’s Modified Eagle’s Medium (DMEM) with glucose and without glucose and all medium additives were obtained from Gibco BRL (Grand Island, NE, USA). Methylthiazolyldiphenyl-tetrazolium bromide (MTT), glucose, metformin, N-acetyl-l-cysteine (NAC), FH535, and 2-7-Dichlorodihydrofluorescein diacetate (DCFDA) fluorescence kit were purchased from Sigma Aldrich (St. Louis, MI, USA). PD98059 was purchased from Calbiochem (La Jolla, CA, USA). LDH, Caspase-3 activity assay kit and ATP measurement kits were purchased from BioVision (Milpitas, CA, USA). The antibodies specific for ERK, p-ERK, GSK3β, p-GSK3β, and PARP were purchased from Cell Signaling Technology (Danvers, MA, USA). The antibodies specific for Bax and Bcl-2 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and the antibody specific for GAPDH was purchased from Thermo Fisher (Rockford, IL, USA).

Lamichhane S., Bastola T., Pariyar R., Lee E.S., Lee H.S., Lee D.H, & Seo J. (2017). ROS Production and ERK Activity Are Involved in the Effects of d-β-Hydroxybutyrate and Metformin in a Glucose Deficient Condition. International Journal of Molecular Sciences, 18(3), 674.

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