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Q500mc software qwin

Manufactured by Leica
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Sourced in Germany

The Q500MC Software‐Qwin is a digital imaging software solution for Leica's Q500MC microscope. It provides tools for image capture, analysis, and processing. The software allows users to view, measure, and quantify features within digital microscope images.

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Lab products found in correlation

Oil red o, by Merck Group (1 mentions) Qiazol lysis reagent, by Qiagen (1 mentions) Q500 mc image analysis system, by Leica (1 mentions) Ez chamber slides, by Merck Group (1 mentions)

Close spelling variants of this product

Qwin software Q500MC

2 protocols using q500mc software qwin

1

Muscle Biopsy and Lipid Analysis

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Muscle biopsies were obtained from rectus abdominis muscle, at the beginning of surgery. Tissue (90 mg) RNA was isolated using the Qiazol Lysis reagent (Qiagen Sciences, Maryland, USA). Isolated RNA was stored at −80 °C until use. Other sample aliquots were used for immunohistochemical staining, and for protein (western blot) analysis. For the study of fat infiltration, tissue samples were stained with Oil‐red‐O, which detect lipids, mainly neutral fats and cholesteryl esters (Sigma‐Aldrich, Milan, Italy) (31). Digital images of immunohistochemical and Oil‐red‐O stained sections were obtained using a Leica microscope (Leica Microsystems GmbH Wetzlar, Germany) equipped with a digital camera controlled by Q500MC Software‐Qwin (Leica).12
Verzola D., Bonanni A., Sofia A., Montecucco F., D'Amato E., Cademartori V., Parodi E.L., Viazzi F., Venturelli C., Brunori G, & Garibotto G. (2016). Toll‐like receptor 4 signalling mediates inflammation in skeletal muscle of patients with chronic kidney disease. Journal of Cachexia, Sarcopenia and Muscle, 8(1), 131-144.
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2

Actin Cytoskeletal Changes in Response to Myostatin

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A7r5 cells grown on EZ-chamber slides (Merckgroup) and freshly isolated monocytes were treated over night with Mstn (500 ng/ml). Cells were fixed with 2% paraformaldehyde, permeabilized with 0.05%Triton X 100, and stained with 5 U/ml Alexa-Fluor 488-conjugated phalloidin (A-12379 Alexa488 phalloidin; Molecular Probes). Cells were washed three times with PBS and viewed by fluorescence microscopy. Images were captured using the Leica Q500 MC image analysis system (Leica). Single images were digitized for image analysis at 256 gray levels. Imported data were quantitatively analyzed using Q500MC Software-Qwin (Leica). Constant optical threshold and filter combination were used.
Verzola D., Milanesi S., Bertolotto M., Garibaldi S., Villaggio B., Brunelli C., Balbi M., Ameri P., Montecucco F., Palombo D., Ghigliotti G., Garibotto G., Lindeman J.H, & Barisione C. (2017). Myostatin mediates abdominal aortic atherosclerosis progression by inducing vascular smooth muscle cell dysfunction and monocyte recruitment. Scientific Reports, 7, 46362.
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