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49 protocols using thiourea

1

Cellulose Extraction from Softwood Pulp

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Cellulose from dissolved softwood pulp was purchased from Shandong Bohai Industry Co., Ltd. (Boxing, China). Sulfuric acid (H2SO4, 98% purity, Kermel Analytical Reagent Co., Ltd., Tianjin, China), rutin (95% purity, Aladdin Chemistry Co., Ltd., Shanghai, China), and thiourea (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China) were used without further purification.
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2

Proteomic Analysis of Bovine Serum

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Albumin from bovine serum was procured from Wuhan Chucheng Zhengmao Science and Technology Engineering Co., Ltd. DL-Dithiothreitol were procured from Solarbio, while EDTA, Xylene brilliant cyaninG-250, Sodium dodecyl sulfate, ThioUrea, and Acetone were procured from Sinopharm. Iodoacetamide was procured from Aladdin, and Phenylmethanesulfonyl fluoride was procured from Xiya Reagent. Tetraethylammonium bromide and Urea were procured from Sigma. Trypsin was procured from Promega, and the protein marker was procured from Fementas. Lastly, the bicinchoninic acid (BCA) protein quantification kit was procured from Biyuntian. ELISA kits for myosin heavy chain 9 (MYH9) and fetuin B (FETUB) were procured from Wuhan Fearn and Eliot Biotechnology Co.
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3

Hydrothermal Synthesis of Multicomponent Nanostructures

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Ferric nitrate nonahydrate (Fe(NO3)3·9H2O, 99% Alfa Aesar), distilled water, Zn(NO3)3·6H2O, 97% Sinopharm, sodium hydroxide (NaOH, 99% Alfa Aesar), polyethylene glycol (PEG, 97% DAEJUNG), MB (C16H18ClN3S, 99% Simpsons), cobalt nitrate hexahydrate (Co(NO3)2·6H2O, 97% DAEJUNG) and thiourea (CH4N2S, 99% Sinopharm).
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4

Corn Stover Pretreatment and Characterization

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Corn stover was collected from Mengcheng City, Anhui Province of China. After being washed and dried at 60 °C for 48 h, the stock was cut and milled. The corn stover fragments sieved through a 30-mesh screen were collected and sealed for later use after being dried in an oven at 105 °C for 6 h. Hydrochloric acid (36–38%), sulfuric acid (95–98%), methanol, water-free ethanol, acetone, ammonium molybdate tetrahydrate, and thiourea were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Methyl sulfoxide-d6 (99.9%) for NMR characterization was purchased from Aladdin (Shanghai, China). All chemicals were used without further purification.
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5

Mesoporous Carbon Synthesis and Characterization

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Formaldehyde (HCHO), sodium molybdate dihydrate (Na2MoO4 2H2O), thiourea (NH2CSNH2), N,N-dimethylformamide (C3H7NO), ethanol absolute (C2H6O), and isopropanol (C3H8O) were bought from Sinopharm Chemical Reagent Co., Ltd (Beijing, China), and 5, 5-dimethyl-1-pyrroline-N-oxide (DMPO) used for radical analysis was provided by Sigma Chemical Co. Ltd. Ordered mesoporous carbon (OMC) was provided by Low-Dimension Materials. The type of OMC was CMK-3 and provided by the Low-Dimension Materials. The specific surface area was 1,000 m2/g, total pore volume was 1.35 cm3/g, and the micropore volume was 0.01 cm3/g. All chemicals used in the experiments were analytically pure and provided by the company without further purification.
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6

Synthesis of Functional Nanomaterials

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Graphite powder, ethylene glycol (EG), polyethylene 2000 (PEG 2000), anhydrous ferric chloride (FeCl3), cobalt(ii) chloride hexahydrate (CoCl2·6H2O), sodium acetate (NaAc), tetrabutyltitanate (TBOT), thiourea, methylene blue, methylene orange, rhodamine B and other reagents were purchased from Sinopharm Group Chemical Reagent Co (China). All chemicals used were analytical grade and were used without further purification.
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7

Synthesis of Multicomponent Nanostructures

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Bismuth nitrate (Bi(NO3)3·5H2O), sodium molybdat (Na2MoO4·2H2O), cadmium acetate (Cd(CH3CO2)2·2H2O), thiourea (CH4N2S), ethylenediamine (EDA), ethylene glycol (EG), chloroplatinic acid hexahydrate (H2PtCl6·6H2O, AR) were purchased from Sinopharm Chemical Reagent Co. Ltd. All chemicals and reagents were used as received from commercial suppliers without further purification, deionized water was used in all experiments.
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8

Synthesis of Inorganic Nanoparticles

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FTO was purchased from Luoyang Guluo Glass Co, (Luoyang, China). Sodium tungstate (Na2WO4·2H2O), sodium molybdate (Na2MoO4·2H2O), and thiourea (CH4N2S) were purchased from Sinopharm Chemical Reagent Co. (Shanghai, China). Sodium chloride (NaCl), concentrated hydrochloric acid (HCl), and glucose (C6H12O6) were purchased from the Tianjin Damao Chemical Reagent Factory (Tianjin, China). The above chemicals were of analytical grade and were used without further purification. Deionised (DI) water was used throughout the study.
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9

Sepiolite Fiber Adsorption Protocol

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The sepiolite fiber was purchased from Neixiang Xinglei Sepiolte Co., Ltd., Nanyang, Henan Province, China. Melamine (AR, 99%), methylene blue (BS) and thiourea (AR, 99%) were purchased from Sinopharm Chemical Reagent Co., Ltd., Shanghai, China. Tert-butanol (AR, 99%) and disodium ethylene diamine tetraacetate (EDTA, AR, 99%) were purchased from Xilong Chemical Co., Ltd., Shantou, China. All the chemical reagents were used without further purification. Distilled water was used throughout the experiment.
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10

Synthesis and Characterization of Pyrrole-Styrene Copolymer

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Pyrrole (C4H5N, CP), styrene (C8H8, >99.5%), amino-1H-tetrazole (CH3N5, >99.0%), sodium dodecyl sulfate (SDS, >99.0%), were purchased from Aladdin Chemistry Co., Ltd. Pyrrole and styrene monomer were distilled under reduced pressure before use. Pt/C (40%, JM) powders (HiSPEC 3000) were obtained from Alfa Aesar. Thiourea (CH4N2S, >99.0%), sodium carbonate (Na2CO3, >98.0%), potassium persulfate (K2S2O8, >99.0%), sodium persulfate ((NH4)2S2O8, >98.0%), polyvinyl alcohol (PVA) were purchased from Sinopharm Chemicals Co., Ltd. Potassium hydroxide (KOH, >95.0%), sulfuric acid (H2SO4, >98.0%), methanol (CH3OH, >99.5%), ethanol (C2H5OH, >99.0%) were provided by Xilong Chemical Reagent Co., Ltd. Distilled deionized water (>18.2 MΩ) was produced by reverse osmosis followed by ion-exchange and filtration (HBI, Shenzhen Honbo Water Treatment Equipment Co., Ltd., China). All chemicals and regents were used without further purification apart from pyrrole and styrene.
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